Analysis of Gene Expression Induced by Irritant and Sensitizing Chemicals Using Oligonucleotide Arrays

Details

• Personal Author:
  He B ; Meade BJ ; Munson AE
• Description:
  Chemical-induced allergy continues to be an important occupational health problem. Despite decades of investigation, the molecular mechanisms underlying chemical-induced hypersensitivity and irritancy remain unclear because of the complex interplay between properties of different chemicals and the immune system. In this study, gene expression induced by toluene diisocyanate (TDI, a primarily IgE-inducing sensitizer), oxazolone (OXA, a cell-mediated hypersensitivity inducing sensitizer), or nonanoic acid (NA, a non-sensitizing irritant) was investigated using gene arrays. Female BALB/c mice were dermally exposed on the ears once daily for 4 consecutive days. On day 5, the lymph nodes draining the exposure sites were collected and used for RNA extraction and subsequent hybridization to Affymetrix Mu6500 oligonucleotide arrays. Of the 6519 genes on the arrays, there were 44, 13, and 51 genes in the TDI-, OXA-, and NA-exposed samples, respectively, that displayed a minimum of twofold change in expression level relative to the vehicle control. There were 32, 19, and 19 genes that were differentially expressed (with a minimum of twofold change) between TDI and OXA, TDI and NA, OXA and NA, respectively. The differentially expressed genes include immune response-related genes, transcriptional factors, signal transducing molecules, and Expressed Sequence Tags. Based on the gene array results, candidate genes were further evaluated using RT-PCR. There was only about 47% concordance between the gene array and RT-PCR results. [Description provided by NIOSH]
• Subjects:
  Animals Dermatitis Environmental Monitoring Genetics Hypersensitivity Immune System Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Exposure-assessment Exposure-levels Genes Immune-reaction Immune-system In-vitro-studies Laboratory-animals Lymph-nodes Occupational-health
• ISSN:
  1567-5769
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  OD - Office of the Director ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  1
• Issue:
  5
• NIOSHTIC Number:
  nn:20032162
• Citation:
  Int Immunopharmacol 2001 May; 1(5):867-879
• CAS Registry Number:
  Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  International Immunopharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ff50fd182bfcc31a9720f0e9c113251104134b2c09eb38869c3e50c2ca0b73a0bb3f41b68b441f4063fc7e86e47cc60e3d009dae1da7542792f835156b252c97
• Download URL:
  https://stacks.cdc.gov/view/cdc/186206/cdc_186206_DS1.pdf
• File Type:
  [PDF - 373.65 KB ]