Restraint-Induced Modulation of Allergic and Irritant Contact Dermatitis in Male and Female B6.129 Mice

Details

• Personal Author:
  Flint MS ; Miller DB ; Tinkle SS
• Description:
  Recent studies in rats have indicated that acute restraint enhances cutaneous hypersensitivity. We hypothesized that acute restraint would also modulate the development of allergic and irritant dermatitis in mice and that these restraint-induced changes would be reflected in the cutaneous cytokine profile and be gender-specific. For these studies, male and female B6.129 mice were sensitized and challenged with the contact sensitizer dinitrofluorobenzene or challenged with the irritant croton oil. Two-hour restraint was applied prior to chemical challenge. Restraint combined with chemical increased ear swelling in both genders in ACD, a change that was blocked by administration of RU-486 prior to restraint. Neither restraint nor RU-486 administration modulated development of ICD; however, IL-1 beta was decreased by restraint in females only. TNF-alpha and IFN-gamma production were modified in ACD; TNF-alpha in both genders and IFN-gamma in female mice only. Our data demonstrate that acute restraint increases serum corticosterone in B6.129 male and female mice to comparable levels. Restraint modulated the murine ear swelling in ACD, but not ICD, in both genders, and the change in the ear swelling response and cytokine production were, at least in part, corticosterone-dependent. [Description provided by NIOSH]
• Subjects:
  Animals Dermatitis Hypersensitivity Immune System Laboratories Occupational Health Safety Skin Skin Diseases
• Keywords:
  Allergens Allergic-dermatitis Allergic-reactions Allergies Animal-studies Contact-allergies Contact-dermatitis Immune-reaction Immune-system Laboratory-animals Sensitization Skin-disorders Skin-irritants Skin-sensitivity

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• ISSN:
  0889-1591
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  256-269
• Volume:
  14
• Issue:
  4
• NIOSHTIC Number:
  nn:20032180
• Citation:
  Brain Behav Immun 2000 Dec; 14(4):256-269
• Contact Point Address:
  NIOSH, Toxicology and Molecular Biology Branch, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, West Virginia, 26505
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Brain, Behavior, and Immunity
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6acb1b9abc911f961809df4d7a290465eb8d1814e26ed9d96e162b628641df7de2131abb502d1e8052a4abe1f0569ecd91ecf1415e777d483f7a50f87492d0cc
• Download URL:
  https://stacks.cdc.gov/view/cdc/186222/cdc_186222_DS1.pdf
• File Type:
  [PDF - 200.03 KB ]