Microarray Analysis of Gene Expression Patterns Induced by Irritant and Sensitizing Chemicals

Details

• Personal Author:
  He B ; Meade BJ ; Munson AE
• Description:
  Chemical-induced dermatitis continues to be an important occupational health problem. Despite decades of investigation, the molecular mechanisms underlying chemical induced hypersensitivity and irritation remain unclear due to the complicated interplay between properties of different chemicals and the immune system. In this study gene expression patterns induced by Toulene Diisocyanate (TDI, IgE-inducing sensitizer) or Nonanoic acid (non-sensitizing irritant) were investigated using gene microarrays. Female BALB/c mice were dermally exposed on the ears once daily for 4 consecutive days. On day 5 the lymph nodes draining the exposure sites were collected and used for mRNA extraction. For TDI and OXA exposures, the concentrations used induced similar quantities of mRNA in the draining lymph nodes. The extracted mRNAs were reverse transcribed into cDNAs and then in vitro transcribed into biotin-labeled cRNAs. The hybridization of labeled cRNAs to GeneChip Mu6500 oligonucleotide arrays (Affymetrix, CA) and scanning were conducted by Research Genetics, Inc. Of the 6,500 genes on the arrays, there were 19 genes whose expression levels were significantly different between TDI- and nonanoic acid-treated samples, 18 genes between OXA and nonanoic acid samples and 33 genes between TDI and OXA samples. These include immune response-related genese, transcriptional factors, signal transducing molecules and Expressed Sequence Tags. Microarray analysis indentified differentially expressed genes which can be further investigated by conventional methods. Candidate genes will be chosen for further evaluation following exposure to a panel of chemical sensitizers and irritants. Further studies will be conducted to define the functional roles of these genes. [Description provided by NIOSH]
• Subjects:
  Animals Dermatitis Environmental Exposure Genetics Hypersensitivity Immune System Laboratories Lymphatic System Occupational Health Safety
• Keywords:
  Animal Studies Exposure Assessment Exposure Levels Genes Immune Reaction Immune System In Vitro Studies Laboratory Animals Lymph Nodes Occupational Health
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  54
• Issue:
  1
• NIOSHTIC Number:
  nn:20020943
• Citation:
  Toxicologist 2000 Mar; 54(1):305
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 39th Annual Meeting, March 19-23, 2000, Philadelphia, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cec2edce8091426e32213d3da623a0d7eee3acb8322e47406926206b894fe08f65382bdb95a3a9f89498111526c870009efc82194d46caf53c4bde97836e694e
• Download URL:
  https://stacks.cdc.gov/view/cdc/198565/cdc_198565_DS1.pdf
• File Type:
  [PDF - 474.94 KB ]