Comparison of NiSO4 and NiCl2 as sensitizers in the guinea pig.

Details

• Personal Author:
  Ahmed W ; Dorn CR ; Warner RD
• Description:
  The effectiveness of nickel-sulfate (7786814) and nickel-chloride (7718549) as skin sensitizers in guinea-pigs was compared, in order to determine which compound was the most appropriate for the guinea-pig maximization test (GPMT). The GPMT was conducted in female Hartley-guinea-pigs using 0, 0.03125, 0.0625, 0.125, 0.25, or 0.5 percent nickel-chloride or nickel-sulfate intradermally or topically as sensitizing doses. Intradermal challenge with the same doses was performed 23 days later, and erythematous reactions were measured. The lymphocyte transformation test (LTT) was conducted in cells from sensitized guinea-pigs using 0.108x10(-6), 2.70x10(-6), or 5.40x10(-6) molar nickel-sulfate or nickel-chloride for comparison purposes. In the GPMT, erythematous responses at the challenge site for 0.125, 0.25, or 0.5 percent nickel-sulfate were greater than for controls when read at 24 and 48 hours. In guinea-pigs treated with nickel-chloride, the response was significantly different from controls only for 0.5 percent nickel-chloride. In the LTT, responses to both nickel-sulfate and nickel-chloride were significantly greater than that of controls. The response to nickel-sulfate was greater than that of nickel-chloride; however, the difference was not significant. The authors conclude that using nickel-sulfate as a sensitizer in the GPMT is preferable to using nickel-chloride, and that skin testing may be more valuable than the LTT for the GPMT with these sensitizers. [Description provided by NIOSH]
• Subjects:
  Blood Dermatitis Hypersensitivity Laboratories Nickel Occupational Health Safety Skin
• Keywords:
  Blood-cells In-vitro-studies In-vivo-studies Laboratory-animals Nickel-compounds NIOSH-Grant NIOSH-Publication Physiological-response Sensitization Skin-tests
• ISSN:
  0020-5915
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  332-336
• Volume:
  85
• Issue:
  3
• NIOSHTIC Number:
  nn:00179664
• Citation:
  Int Arch Allergy Appl Immunol 1988 Jan; 85(3):332-336
• Contact Point Address:
  Veterinary Preventive Medicine Ohio State University 1900 Coffey Road Columbus, Ohio 43210
• CAS Registry Number:
  Nickel dichloride (CAS RN 7718-54-9) ; Nickel sulfate (NiSO4) (CAS RN 7786-81-4)
• Federal Fiscal Year:
  1988
• Performing Organization:
  Ohio State University, Columbus, Ohio
• Peer Reviewed:
  True
• Start Date:
  19850927
• Source Full Name:
  International Archives of Allergy and Applied Immunology
• End Date:
  19860831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cff0fcc155065ab4297e98b8994684badaf9599c768e060834da64e0c6cff66c29b01e4469f79e4f9d3938a8ba3e95041f82f2d558f574a0cc1c987beaecd0a2
• Download URL:
  https://stacks.cdc.gov/view/cdc/185517/cdc_185517_DS1.pdf
• File Type:
  [PDF - 2.32 MB ]