Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Söderlund-Venermo, Maria ; Lahtinen, Anne ; Jartti, Tuomas ; Hedman, Lea ; Kemppainen, Kaisa ; ... More +
Söderlund-Venermo, Maria ; Lahtinen, Anne ; Jartti, Tuomas ; Hedman, Lea ; Kemppainen, Kaisa ; Lehtinen, Pasi ; Allander, Tobias ; Ruuskanen, Olli ; Hedman, Klaus Less -
Description:
Human bocavirus (HBoV) is a widespread respiratory virus. To improve diagnostic methods, we conducted immunoglobulin (Ig) G and IgM enzyme immunoassays with recombinant virus-like particles of HBoV as antigen. Acute-phase and follow-up serum samples from 258 wheezing children and single serum samples from 115 healthy adults in Finland were examined. Our assays had a sensitivity of 97% and a specificity of 99.5%. Of adults, 96% had immunity; none had an acute infection. Of 48 children with serologically diagnosed acute HBoV infections, 45 were viremic and 35 had virus in nasopharyngeal aspirates (NPAs). Of 39 HBoV NPA PCR-positive children co-infected with another virus, 64% had a serologically verified HBoV infection. HBoV caused illness of longer duration than rhinovirus and of equal severity to that of respiratory syncytial virus. Among children with bronchiolitis, >25% had acute HBoV infections. Accurate HBoV diagnosis requires serologic analysis or PCR of serum; PCR of NPAs alone is insufficient.
Subjects:
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Antibodies, Viral Antigens, Viral Child Child, Preschool Female Human Bocavirus Humans Immunoglobulin G Immunoglobulin M Infant Male Nasopharynx Parvoviridae Infections Polymerase Chain Reaction Recombinant Proteins Research Respiratory Sounds Virion
Source:
Emerg Infect Dis. 15(9):1423-1430.
Document Type:
Journal Article
Place as Subject:
Finland
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:b706dc302016bca7961736d5ca165a9b71b722258b9228fdea79067018fc2279
Download URL:
https://stacks.cdc.gov/view/cdc/17849/cdc_17849_DS1.pdf
File Type:

Supporting Files

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