The added value of serologic testing: A comparison of influenza incidence among pregnant persons based on molecular-based surveillance versus serologic testing

Details

• Alternative Title:
  Int J Infect Dis
• Personal Author:
  Kittikraisak, Wanitchaya ; Tinoco, Yeny ; Levine, Min Z. ; Mott, Joshua A. ; Kanjanapattanakul, Wiboon ; Munayco, Cesar ; Rawangban, Boonsong ; Hunt, Danielle Rentz ; Mohanty, Sarita ; Wesley, Meredith ; Soto, Giselle ; Florian, Richard ; Gonzales, Oswaldo ; Cabrera, Santiago ; Llajaruna, Edwin ; Asavapiriyanont, Suvanna ; Ellison, Damon W. ; Malek, Parker ; Azziz-Baumgartner, Eduardo ; Dawood, Fatimah S.
• Description:
  Background:
  
  We examined the added value of serologic testing for estimating influenza virus infection incidence based on illness surveillance with molecular testing versus periodic serologic testing.
  
  Methods:
  
  Pregnant persons unvaccinated against influenza at <28 weeks gestation were enrolled before the 2017 and 2018 influenza seasons in Peru and Thailand. Blood specimens were collected at enrollment and ≤14 days postpartum for testing by hemagglutination inhibition assay for antibodies against influenza reference viruses. Seroconversion was defined as a ≥4-fold rise in antibody titers from enrollment to postpartum with the second specimen’s titer of ≥40. Throughout pregnancy, participants responded to twice weekly surveillance contacts asking about influenza vaccination and influenza-like symptoms (ILS). A mid-turbinate swab was collected with each ILS episode for influenza real-time reverse transcription polymerase chain reaction (rRT-PCR).
  
  Results:
  
  Of 1,466 participants without evidence of influenza vaccination during pregnancy, 296 (20.2%) had evidence of influenza virus infections. Fifteen (5.1%) were detected by rRT-PCR only, 250 (84.4%) by serologic testing only, and 31 (10.5%) by both methods.
  
  Conclusions:
  
  Influenza virus infections during pregnancy occurred in 20% of cohort participants; >80% were not detected by a broad illness case definition coupled with rRT-PCR.
  
  
• Subjects:
  Adolescent Adult Antibodies, Viral Female Hemagglutination Inhibition Tests Humans Incidence Influenza, Human Influenza Vaccines Peru Pregnancy Pregnancy Complications, Infectious Serologic Tests Thailand Young Adult

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• Keywords:
  Influenza Serology
• Source:
  Int J Infect Dis. 149:107264
• Pubmed ID:
  39426491
• Pubmed Central ID:
  PMC11710853
• Document Type:
  Journal Article
• Funding:
  U01 GH002084/GH/CGH CDC HHSUnited States/
• Volume:
  149
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:f8f84b0f2a35350b979066233fa8e7d48241657d9bafa6eaaa7d2e566e9be7f8d1ee8dd6f5b185189da721c2f91d79e85aa749f0084b5a2868b12bee79c51a74
• Download URL:
  https://stacks.cdc.gov/view/cdc/175584/cdc_175584_DS1.pdf
• File Type:
  [PDF - 437.00 KB ]