Bacterial Phenotype Variants in Group B Streptococcal Toxic Shock Syndrome1

Sendi, Parham; Johansson, Linda; Dahesh, Samira; Van Sorge, Nina M.; Darenberg, Jessica; Norgren, Mari; Sjölin, Jan; Nizet, Victor; Norrby-Teglund, Anna

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Bacterial Phenotype Variants in Group B Streptococcal Toxic Shock Syndrome1

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Feb 2009
By Sendi, Parham ; Johansson, Linda ; Dahesh, Samira ; ...

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Alternative Title:

Emerg Infect Dis
Personal Author:

Sendi, Parham ; Johansson, Linda ; Dahesh, Samira ; Van Sorge, Nina M. ; Darenberg, Jessica ; Norgren, Mari ; Sjölin, Jan ; Nizet, Victor ; Norrby-Teglund, Anna
Description:

We conducted genetic and functional analyses of isolates from a patient with group B streptococcal (GBS) necrotizing fasciitis and toxic shock syndrome. Tissue cultures simultaneously showed colonies with high hemolysis (HH) and low hemolysis (LH). Conversely, the HH and LH variants exhibited low capsule (LC) and high capsule (HC) expression, respectively. Molecular analysis demonstrated that the 2 GBS variants were of the same clonal origin. Genetic analysis found a 3-bp deletion in the covR gene of the HH/LC variant. Functionally, this isolate was associated with an increased growth rate in vitro and with higher interleukin-8 induction. However, in whole blood, opsonophagocytic and intracellular killing assays, the LH/HC phenotype demonstrated higher resistance to host phagocytic killing. In a murine model, LH/HC resulted in higher levels of bacteremia and increased host mortality rate. These findings demonstrate differences in GBS isolates of the same clonal origin but varying phenotypes.
Subjects:

Animals Bacterial Capsules Bacterial Proteins Beta-hemolysin Carotenoid Pigment Fasciitis, Necrotizing Genetic Variation Group B Streptococci Hemolysis Humans Male Mice Middle Aged Neutrophils Opsonin Proteins Phagocytosis Phenotype Repressor Proteins Research Shock, Septic Streptococcal Infections Streptococcus Agalactiae Toxic Shock Syndrome
Source:

Emerg Infect Dis. 15(2):223-232.
Document Type:

Journal Article
Volume:

15
Issue:

2
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:209a7551acb32bddb3f43b0bb9972c573f2768fa380dff9b99734f30f271b978
Download URL:

https://stacks.cdc.gov/view/cdc/17404/cdc_17404_DS1.pdf
File Type:

[PDF - 570.75 KB ]

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