Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Hofmann, Martin A. ; Renzullo, Sandra ; Mader, Markus ; Chaignat, Valérie ; Worwa, Gabriella ; ... More +
Hofmann, Martin A. ; Renzullo, Sandra ; Mader, Markus ; Chaignat, Valérie ; Worwa, Gabriella ; Thuer, Barbara ; Less -
Description:
A novel bluetongue virus (BTV) termed Toggenburg orbivirus (TOV) was detected in goats from Switzerland by using real-time reverse transcription-PCR. cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplified by PCR and cloned into a plasmid vector. Five clones for each genome segment were sequenced to determine a consensus sequence. BLAST analysis and dendrogram construction showed that TOV is closely related to BTV, although some genome segments are distinct from the 24 known BTV serotypes. Maximal sequence identity to any BTV ranged from 63% (segment 2) to 79% (segments 7 and 10). Because the gene encoding outer capsid protein 2 (VP2), which determines the serotype of BTV, is placed within the BTV serogroup, we propose that TOV represents an unknown 25th serotype of BTV.
Subject:
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Animals
Bluetongue Virus
DNA, Complementary
Goats
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Research
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Serotyping

Source:
Emerg Infect Dis. 14(12):1855-1861 ;
Pubmed ID:
19046507
Pubmed Central ID:
PMC2634640
Document Type:
Journal Article ;
Place as Subject:
Switzerland ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:3a0a9f0d1feb9ca4ec483666e058cefd21b2c8632b5fcbb1619bc337f4f86ae1
File Type:

Supporting Files

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