Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Botha, Elizabeth M. ; Markotter, Wanda ; Wolfaardt, Mariaan ; Paweska, Janusz T. ; Swanepoel, Robert ; ... More +
Botha, Elizabeth M. ; Markotter, Wanda ; Wolfaardt, Mariaan ; Paweska, Janusz T. ; Swanepoel, Robert ; Palacios, Gustavio ; Nel, Louis H. ; Venter, Marietjie ; Less -
Description:
We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3 noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study.
Subject:
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Animals
Genetic Variation
Genome, Viral
Genotype
Humans
Mice
Molecular Sequence Data
Mutation
Phylogeny
Research
Sequence Analysis, DNA
Viral Envelope Proteins
Viral Nonstructural Proteins
Virulence
West Nile Fever

Source:
Emerg Infect Dis. 14(2):222-230. ;
Document Type:
Journal Article ;
Place as Subject:
South Africa ;
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:445129622b57d8eca8fb2afb28e862390f9893b78fc952e65925e93c43311b82
File Type:

Supporting Files

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  	07-0457_finalR.nxml	txt
  	license.txt	txt

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