Content
and Copyright
i
In Vitro Cell Culture Infectivity Assay for Human Noroviruses
-
Published Date:
Mar 2007
Source:Emerg Infect Dis. 13(3):396-403.
[PDF-388.74 KB]
Details:
-
Alternative Title:Emerg Infect Dis
-
Personal Author:
-
Description:Human noroviruses cause severe, self-limiting gastroenteritis that typically lasts 24-48 hours. Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. We show, for the first time, that noroviruses can infect and replicate in a physiologically relevant 3-dimensional (3-D), organoid model of human small intestinal epithelium. This level of cellular differentiation was achieved by growing the cells on porous collagen-I coated microcarrier beads under conditions of physiological fluid shear in rotating wall vessel bioreactors. Microscopy, PCR, and fluorescent in situ hybridization provided evidence of norovirus infection. Cytopathic effect and norovirus RNA were detected at each of the 5 cell passages for genogroup I and II viruses. Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed.
-
Subject:
-
Document Type:
-
Collection(s):
-
Main Document Checksum:
- File Type:
-
Supporting Files:
image/gif 
image/jpeg 
text/plain
text/plain 
image/gif 
image/jpeg 
image/gif 
image/jpeg 
image/gif 
image/jpeg 
image/gif 
image/jpeg
No Related Documents.
You May Also Like:
-
Published Date:Source:Description:File Type:[PDF - 1.18 MB]
File Type:[PDF - 1.18 MB]
Published Date:Source:File Type:[PDF - 102.68 KB]
File Type:[PDF - 102.68 KB]
Published Date:Source:Description:File Type:[PDF - 219.27 KB]
File Type:[PDF - 219.27 KB]
Published Date:Source:Description:File Type:[PDF - 244.46 KB]
File Type:[PDF - 244.46 KB]
Published Date:Source:Description:File Type:[PDF - 180.79 KB]
File Type:[PDF - 180.79 KB]
Have Questions?
Visit CDC Info
Email
CDC-INFO
Open 24/7
CDC INFORMATION
CONNECT WITH CDC
