Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Straub, Timothy M. ; Höner zu Bentrup, Kerstin ; Coghlan, Patricia Orosz ; Dohnalkova, Alice ; Mayer, Brooke K. ; ... More +
Straub, Timothy M. ; Höner zu Bentrup, Kerstin ; Coghlan, Patricia Orosz ; Dohnalkova, Alice ; Mayer, Brooke K. ; Bartholomew, Rachel A. ; Valdez, Catherine O. ; Bruckner-Lea, Cynthia J. ; Gerba, Charles P. ; Abbaszadegan, Morteza A. ; Nickerson, Cheryl A. Less -
Description:
Human noroviruses cause severe, self-limiting gastroenteritis that typically lasts 24-48 hours. Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. We show, for the first time, that noroviruses can infect and replicate in a physiologically relevant 3-dimensional (3-D), organoid model of human small intestinal epithelium. This level of cellular differentiation was achieved by growing the cells on porous collagen-I coated microcarrier beads under conditions of physiological fluid shear in rotating wall vessel bioreactors. Microscopy, PCR, and fluorescent in situ hybridization provided evidence of norovirus infection. Cytopathic effect and norovirus RNA were detected at each of the 5 cell passages for genogroup I and II viruses. Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed.
Subject:
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3-dimensional Cell Culture
Caliciviridae Infections
Cell Culture Techniques
Cell Line
Collagen Type I
Cytopathic Effect
Cytopathogenic Effect, Viral
Electron Microscopy
Fluorescence
Humans
Intestinal Mucosa
Microspheres
Norovirus
Organoids
Research
RNA, Viral
RWV Bioreactor
Norovirus

Source:
Emerg Infect Dis. 13(3):396-403.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:3620560172544459f1b57199e9d68a93a8cd77f980e8ca046228217842c90c81
File Type:

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