Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Johnson, D. J. ; Ostlund, E. N. ; Pedersen, D. D. ; Schmitt, B. J.
• Description:
  A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.
• Subjects:
  Animals Bird Diseases Birds Brain Disease Reservoirs Horse Diseases Horses Reverse Transcriptase Polymerase Chain Reaction Rna, Viral Sensitivity And Specificity West Nile Fever West Nile Virus

  More +
• Source:
  Emerg Infect Dis. 7(4):739-741.
• Document Type:
  Journal Article
• Place as Subject:
  New York ; North America
• Location:
  North America
• Volume:
  7
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:0dcbbccbcbc89a05184a74783f4001998c1b4a2123d0e3ff5a79ea42dbde6ab6
• Download URL:
  https://stacks.cdc.gov/view/cdc/15191/cdc_15191_DS1.pdf
• File Type:
  [PDF - 56.05 KB ]