Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

Johnson, D. J.; Ostlund, E. N.; Pedersen, D. D.; Schmitt, B. J.

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CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

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Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

2001 Jul-Aug
By Johnson, D. J. ; Ostlund, E. N. ; Pedersen, D. D. ; ...
Source: Emerg Infect Dis. 7(4):739-741.

[PDF-56.05 KB]

English

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Johnson, D. J. ; Ostlund, E. N. ; Pedersen, D. D. ; Schmitt, B. J.

Johnson, D. J. ; Ostlund, E. N. ; Pedersen, D. D. ; Schmitt, B. J. Less -
Description:

A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.
Subjects:

[+]

Animals Bird Diseases Birds Brain Disease Reservoirs Horse Diseases Horses Reverse Transcriptase Polymerase Chain Reaction Rna, Viral Sensitivity And Specificity West Nile Fever West Nile Virus
Source:

Emerg Infect Dis. 7(4):739-741.
Document Type:

Journal Article
Place as Subject:

New York ; North America

New York ; North America Less -
Location:

North America
Volume:

7
Issue:

4
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:0dcbbccbcbc89a05184a74783f4001998c1b4a2123d0e3ff5a79ea42dbde6ab6
Download URL:

https://stacks.cdc.gov/view/cdc/15191/cdc_15191_DS1.pdf
File Type:

	11585541.nxml	txt
	license.txt	txt

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