An ex vivo avian leukocyte culture model for West Nile virus infection

Dietrich, Elizabeth A.; Bowen, Richard A.; Brault, Aaron C.

doi:10.1016/j.jviromet.2015.03.004

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An ex vivo avian leukocyte culture model for West Nile virus infection

Published Date:

Mar 14 2015

Publisher's site:

http://dx.doi.org/10.1016/j.jviromet.2015.03.004 New Site Icon

Source:

J Virol Methods. 218:19-22.

[PDF-531.22 KB]

Details:

Personal Authors:

Dietrich, Elizabeth A. ; Bowen, Richard A. ; Brault, Aaron C. ;

Keywords:

[+]

Pubmed ID:

25783683

Pubmed Central ID:

PMC4583197

Description:

West Nile virus (WNV) replicates in a wide variety of avian species, which act as amplification hosts. In particular, WNV generates high titers and elicits severe pathology in American crows (AMCRs; Corvus brachyrhynchos), a species that has been used as a sentinel for WNV transmission. Although the specific cellular targets of WNV replication in AMCRs are not well defined, preliminary evidence suggests that leukocytes may be an important target of early replication. Therefore, development of a protocol for ex vivo culture of AMCR leukocytes as a model for assessing differential avian host susceptibility is described herein. WNV growth in these cultures mirrored in vivo viremia profiles. These data indicate that ex vivo leukocyte cultures can be used for preliminary pathological assessment of novel WNV strains and potentially of other flaviviruses that use avian reservoir hosts.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

CC999999/Intramural CDC HHS/United States

Supporting Files:

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