Partial genetic characterization of West Nile virus strains, New York State, 2000.
Supporting Files
Public Domain
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2001 Jul-Aug
File Language:
English
Details
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Alternative Title:Emerg Infect Dis
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Personal Author:Ebel, G. D. ; Dupuis, A. P. ; Ngo, K. ; Nicholas, D. ; Kauffman, E. ; Jones, S. A. ; Young, D. ; Maffei, J. ; Shi, P. Y. ; Bernard, K. ; Kramer, L. D.
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Description:We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.
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Subjects:
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Source:Emerg Infect Dis. 7(4):650-653.
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Document Type:
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Place as Subject:
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Volume:7
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Issue:4
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Main Document Checksum:urn:sha256:275c091706c3a09f5e42a31db680c2b6ab0fdc7b49e32d8bc6e067f3570a9255
Supporting Files
File Language:
English
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Emerging Infectious Diseases