Details:

Alternative Title:
Virology
Personal Author:
Worwa, Gabriella ; Hutton, Andra A. ; Frey, Michèle ; Duggal, Nisha K. ; Brault, Aaron C. ; ... More +
Worwa, Gabriella ; Hutton, Andra A. ; Frey, Michèle ; Duggal, Nisha K. ; Brault, Aaron C. ; Reisen, William K. Less -
Description:
To investigate the phenotypic evolution of West Nile virus (WNV) in California, we competed sixteen isolates made during 2007-08 against COAV997-5nt, a genetically marked clone from the founding 2003 California isolate COAV997-2003. Using in vivo fitness competitions in House Finches (HOFI) and Culex tarsalis mosquitoes, we found that the majority of WNV WN02 and SW03 genotype isolates exhibited elevated replicative fitness in both hosts compared to COAV997-5nt. Increased replicative capacity in HOFIs was not associated with increased mortality, indicating that these isolates had not gained avian virulence. One WN02 isolate from Coachella Valley, a region geographically close to the isolation of COAV997, showed neutral fitness in HOFIs and reduced fitness in Cx. tarsalis. Two isolates from Kern County and Sacramento/Yolo County out-competed COAV997-nt in HOFIs, but were transmitted less efficiently by Cx. tarsalis. Competition demonstrated neutral or increased fitness that appeared independent of both WN02 and SW03 genotypes.
Subjects:
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Animals Article California Competitive Fitness Culex Evolution Finches Genotype Humans Insect Vectors Invasion Open Reading Frames Phenotype Phylogeny Virus Replication West Nile Fever West Nile Virus
Source:
Virology. 514:170-181
Pubmed ID:
29195094
Pubmed Central ID:
PMC5766374
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States ; R01 AI055607/AI/NIAID NIH HHS/United States
CC999999/Intramural CDC HHS/United States ; R01 AI055607/AI/NIAID NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d9c8715a87d85c8db96d40d75c90974a5a6b47bbc9f9d16c60bdd05bcf2361ac
Download URL:
https://stacks.cdc.gov/view/cdc/50871/cdc_50871_DS1.pdf
File Type:

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