Increases in the competitive fitness of West Nile virus isolates after introduction into California

Worwa, Gabriella; Hutton, Andra A.; Frey, Michèle; Duggal, Nisha K.; Brault, Aaron C.; Reisen, William K.

doi:10.1016/j.virol.2017.11.017

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Increases in the competitive fitness of West Nile virus isolates after introduction into California

Published Date:

Nov 28 2017

Publisher's site:

http://dx.doi.org/10.1016/j.virol.2017.11.017 New Site Icon

Source:

Virology. 514:170-181

Language:

English

[PDF-1001.40 KB]

Details:

Personal Authors:

Worwa, Gabriella ; Hutton, Andra A. ; Frey, Michèle ; Duggal, Nisha K. ; Brault, Aaron C. ; ... More ▼

Keywords:

[+]

Pubmed ID:

29195094

Pubmed Central ID:

PMC5766374

Description:

To investigate the phenotypic evolution of West Nile virus (WNV) in California, we competed sixteen isolates made during 2007-08 against COAV997-5nt, a genetically marked clone from the founding 2003 California isolate COAV997-2003. Using in vivo fitness competitions in House Finches (HOFI) and Culex tarsalis mosquitoes, we found that the majority of WNV WN02 and SW03 genotype isolates exhibited elevated replicative fitness in both hosts compared to COAV997-5nt. Increased replicative capacity in HOFIs was not associated with increased mortality, indicating that these isolates had not gained avian virulence. One WN02 isolate from Coachella Valley, a region geographically close to the isolation of COAV997, showed neutral fitness in HOFIs and reduced fitness in Cx. tarsalis. Two isolates from Kern County and Sacramento/Yolo County out-competed COAV997-nt in HOFIs, but were transmitted less efficiently by Cx. tarsalis. Competition demonstrated neutral or increased fitness that appeared independent of both WN02 and SW03 genotypes.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

CC999999/Intramural CDC HHS/United States
R01 AI055607/AI/NIAID NIH HHS/United States

Supporting Files:

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