Rapid Antigen-Capture Assay To Detect West Nile Virus in Dead Corvids

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Lindsay, Robbin ; Barker, Ian ; Nayar, Gopi ; Drebot, Michael ; Calvin, Sharon ; Scammell, Cherie ; Sachvie, Cheryl ; La Fleur, Tracy Scammell ; Dibernardo, Antonia ; Andonova, Maya ; Artsob, Harvey
• Description:
  The utility of the VecTest antigen-capture assay to detect West Nile virus (WNV) in field-collected dead corvids was evaluated in Manitoba and Ontario, Canada, in 2001 and 2002. Swabs were taken from the oropharynx, cloaca, or both of 109 American Crows, 31 Blue Jays, 6 Common Ravens, and 4 Black-billed Magpies from Manitoba, and 255 American Crows and 28 Blue Jays from Ontario. The sensitivity and specificity of the antigen-capture assay were greatest for samples from American Crows; oropharyngeal swabs were more sensitive than cloacal swabs, and interlaboratory variation in the results was minimal. The sensitivity and specificity of the VecTest using oropharyngeal swabs from crows were 83.9% and 93.6%, respectively, for Manitoba samples and 83.3% and 95.8%, respectively, for Ontario birds. The VecTest antigen-capture assay on oropharyngeal secretions from crows is a reliable and rapid diagnostic test that appears suitable for incorporation into a WNV surveillance program.
• Subjects:
  Animals Birds Corvids Diagnostics Manitoba Ontario Oropharynx Population Surveillance Research Species Specificity Surveillance West Nile Virus Wicking Assay WNV

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• Source:
  Emerg Infect Dis. 9(11):1406-1410.
• Document Type:
  Journal Article
• Volume:
  9
• Issue:
  11
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:2ef99bfe9304947c8f357fc010b4307001b5a902900459e01eef73b023614867
• Download URL:
  https://stacks.cdc.gov/view/cdc/14338/cdc_14338_DS1.pdf
• File Type:
  [PDF - 324.77 KB ]