Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid0912.030008
Personal Author:
Salomone, Oscar A. ; Basquiera, Ana L. ; Sembaj, Adela ; Aguerri, Ana M. ; Reyes, María E. ; ... More +
Salomone, Oscar A. ; Basquiera, Ana L. ; Sembaj, Adela ; Aguerri, Ana M. ; Reyes, María E. ; Omelianuk, Mirtha ; Fernández, Ruth A. ; Enders, Julio ; Palma, Atilio ; Barral, José Moreno ; Madoery, Roberto J. Less -
Description:
Current diagnosis of chronic Chagas disease relies on serologic detection of specific immunoglobulin G against Trypanosoma cruzi. However, the presence of parasites detected by polymerase chain reaction (PCR) in patients without positive conventional serologic testing has been observed. We determined the prevalence and clinical characteristics of persons with seronegative results and T. cruzi DNA detected by PCR in a population at high risk for chronic American trypanosomiasis. We studied a total of 194 persons from two different populations: 110 patients were recruited from an urban cardiology clinic, and 84 persons were citizens from a highly disease-endemic area. Eighty (41%) of persons had negative serologic findings; 12 (15%) had a positive PCR. Three patients with negative serologic findings and positive PCR results had clinical signs and symptoms that suggested Chagas cardiomyopathy. This finding challenges the current recommendations for Chagas disease diagnosis, therapy, and blood transfusion policies.
Subject:
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Adolescent
Adult
Aged
Animals
Antibodies, Protozoan
Argentina
Chagas Disease
Cross-Sectional Studies
Diagnosis
DNA, Protozoan
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Hemagglutination Inhibition Tests
Humans
Male
Middle Aged
Polymerase Chain Reaction
Research
Rural Population
Seroepidemiologic Studies
Trypanosoma Cruzi
Urban Population
Chagas Disease

Source:
Emerg Infect Dis. 9(12):1558-1562.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:eca9cf58d6fa18c67dcd0edb52a99920a09eadaf15be1d326e22694f7f8605b1
File Type:

Supporting Files

• 	03-0008-F.gif	gif
  	03-0008-F.jpg	jpeg
  	03-0008.nxml	txt
  	license.txt	txt

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