Details:

Alternative Title:
mBio
Personal Author:
Worley, Jay Noboru ; Javkar, Kiran ; Hoffmann, Maria ; Hysell, Kristen ; Garcia-Williams, Amanda ; ... More +
Worley, Jay Noboru ; Javkar, Kiran ; Hoffmann, Maria ; Hysell, Kristen ; Garcia-Williams, Amanda ; Tagg, Kaitlin ; Kanjilal, Sanjat ; Strain, Errol ; Pop, Mihai ; Allard, Marc ; Francois Watkins, Louise ; Bry, Lynn Less -
Description:
Multidrug-resistant (MDR) | infections have been identified globally among men who have sex with men (MSM). The highly drug-resistant phenotype often confounds initial antimicrobial therapy, placing patients at risk for adverse outcomes, the development of more drug-resistant strains, and additional treatment failures. New macrolide-resistant | strains complicate treatment further as azithromycin is a next-in-line antibiotic for MDR strains, and an antibiotic-strain combination confounded by gaps in validated clinical breakpoints for clinical laboratories to interpret macrolide resistance in | We present the first high-resolution genomic analyses of 2,097 U.S. | isolates, including those from MDR outbreaks. A sentinel shigellosis case in an MSM patient revealed a strain carrying 12 plasmids, of which two carried known resistance genes, the pKSR100-related plasmid pMHMC-004 and spA-related plasmid pMHMC-012. Genomic-epidemiologic analyses of isolates revealed high carriage rates of pMHMC-004 predominantly in U.S. isolates from men and not in other demographic groups. Isolates genetically related to the sentinel case further harbored elevated numbers of unique replicons, showing the receptivity of this | lineage to plasmid acquisition. Findings from integrated genomic-epidemiologic analyses were leveraged to direct targeted clinical actions to improve rapid diagnosis and patient care and for public health efforts to further reduce spread.| Multidrug-resistant | isolates with resistance to macrolides are an emerging public health threat. We define a plasmid/pathogen complex behind infections seen in the United States and globally in vulnerable patient populations and identify multiple outbreaks in the United States and evidence of intercontinental transmission. Using new tools and sequence information, we experimentally identify the drivers of antibiotic resistance that complicate patient treatment to facilitate improvements to clinical microbiologic testing for their detection. We illustrate the use of these methods to support multiagency efforts to combat multidrug-resistant | using publicly available tools, existing genomic data, and resources in clinical microbiology and public health laboratories to inform credible actions to reduce spread.
Subjects:
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Anti-Bacterial Agents Drug Resistance, Multiple, Bacterial Dysentery, Bacillary Female Genome, Bacterial Homosexuality, Male Humans Internationality Male Microbial Sensitivity Tests Middle Aged Plasmids
Source:
mBio. 12(1)
Pubmed ID:
33500335
Pubmed Central ID:
PMC7858064
Document Type:
Journal Article
Funding:
P30 DK034854/DK/NIDDK NIH HHSUnited States/ ; T32 AI007433/AI/NIAID NIH HHSUnited States/
P30 DK034854/DK/NIDDK NIH HHSUnited States/ ; T32 AI007433/AI/NIAID NIH HHSUnited States/ Less -
Place as Subject:
United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:af707de7ce66f51c508ff605b93c22fbd240b9b4f3eedcbf71ec8dcc19fe3c9a
Download URL:
https://stacks.cdc.gov/view/cdc/111616/cdc_111616_DS1.pdf
File Type:

Supporting Files

• 	mBio.03188-20-sd001.xlsx	xlsx
  	mBio.03188-20-sd002.xlsx	xlsx
  	mBio.03188-20-sd003.pdf	pdf
  	mBio.03188-20-sd004.pdf	pdf
  	mBio.03188-20-sf001.pdf	pdf
  	mBio.03188-20-sf002.pdf	pdf
  	mBio.03188-20-sf003.pdf	pdf
  	mBio.03188-20-st001.docx	docx
  	mBio.03188-20-st002.docx	docx
  	mBio.03188-20-st003.docx	docx
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  	mBio.03188-20.nxml	xml
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