Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

Anh, Nguyen To; Nhu, Le Nguyen Truc; Hong, Nguyen Thi Thu; Phuc, Tran My; Tam, Pham Thi Thanh; Huong, Dang Thao; Anh, Tran Tuan; Deng, Xutao; Nghia, Ho Dang Trung; Nguyen, Tran Thua; Van Hung, Nguyen; Thuan, Nguyen Dac; Phuong, Pham Thi Hong; Chau, Nguyen Van Vinh; Baker, Stephen; Delwart, Eric; Thwaites, Guy; Van Tan, Le

Back to Previous Page

Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

Published Date:

Jan 2021

Source:

Emerg Infect Dis. 27(1):205-213

Language:

English

[PDF-1.78 MB]

Details:

Alternative Title:

Emerg Infect Dis

Personal Author:

Anh, Nguyen To ; Nhu, Le Nguyen Truc ; Hong, Nguyen Thi Thu ; Phuc, Tran My ; Tam, Pham Thi Thanh ; ... More ▼

Corporate Authors:

for the VIZIONS Consortium1

Description:

Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections.

Subject:

[+]

Pubmed ID:

33350920

Pubmed Central ID:

PMC7774551

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Main Document Checksum:

[+]

File Type:

Supporting Files:

	20-2723-Techapp-s1.pdf	application/pdf
	20-2723.nxml	text/xml
	20-2723-F1.gif	image/gif
	20-2723-F1.jpg	image/jpeg
	20-2723-F2.gif	image/gif
	20-2723-F2.jpg	image/jpeg
	20-2723-F3.gif	image/gif
	20-2723-F3.jpg	image/jpeg
	20-2723-F4.gif	image/gif
	20-2723-F4.jpg	image/jpeg

No Related Documents.

Use of Next-Generation Sequencing for Diagnosis of West Nile Virus Infection in Patient Returning to Belgium from Hungary

[PDF - 484.28 KB]

Personal Author:

Wollants, Elke ; Smolders, David ; ...

Published Date:

December 2018

Source:

Emerg Infect Dis. 24(12):2380-2382

Description:

An elderly patient in Belgium who became critically ill after returning from Hungary was tested for pathogens using routine diagnostic tests. All resu...

File Type:

[PDF - 484.28 KB]

File Type:

[PDF - 484.28 KB]
Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

[PDF - 700.76 KB]

Personal Author:

Zhao, Jiangqin ; Ragupathy, Viswanath ; ...

Published Date:

Mar 2015

Source:

Emerg Infect Dis. 21(3):400-408.

Description:

Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform ...

File Type:

[PDF - 700.76 KB]

File Type:

[PDF - 700.76 KB]
Nipah Virus Sequences from Humans and Bats during Nipah Outbreak, Kerala, India, 2018

[PDF - 1.40 MB]

Personal Author:

Yadav, Pragya D. ; Shete, Anita M. ; ...

Published Date:

May 2019

Source:

Emerg Infect Dis. 25(5):1003-1006

Description:

We retrieved Nipah virus (NiV) sequences from 4 human and 3 fruit bat (Pteropus medius) samples from a 2018 outbreak in Kerala, India. Phylogenetic an...

File Type:

[PDF - 1.40 MB]

File Type:

[PDF - 1.40 MB]
Novel Avian Coronavirus and Fulminating Disease in Guinea Fowl, France

[PDF - 510.51 KB]

Personal Author:

Liais, Etienne ; Croville, Guillaume ; ...

Published Date:

Jan 2014

Source:

Emerg Infect Dis. 20(1):105-108.

Description:

For decades, French guinea fowl have been affected by fulminating enteritis of unclear origin. By using metagenomics, we identified a novel avian gamm...

File Type:

[PDF - 510.51 KB]

File Type:

[PDF - 510.51 KB]
Discovery and Characterization of Bukakata orbivirus (Reoviridae:Orbivirus), a Novel Virus from a Ugandan Bat

[PDF - 3.99 MB]

Personal Author:

Fagre, Anna C. ; Lee, Justin S. ; ...

Published Date:

March 02 2019

Source:

Viruses. 2019; 11(3)

Description:

While serological and virological evidence documents the exposure of bats to medically-important arboviruses, their role as reservoirs or amplifying h...

File Type:

[PDF - 3.99 MB]

File Type:

[PDF - 3.99 MB]

Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

You May Also Like:

Have Questions?

CDC INFORMATION

CONNECT WITH CDC