Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Anh, Nguyen To ; Nhu, Le Nguyen Truc ; Hong, Nguyen Thi Thu ; Phuc, Tran My ; Tam, Pham Thi Thanh ; Huong, Dang Thao ; Anh, Tran Tuan ; Deng, Xutao ; Nghia, Ho Dang Trung ; Nguyen, Tran Thua ; Van Hung, Nguyen ; Thuan, Nguyen Dac ; Phuong, Pham Thi Hong ; Chau, Nguyen Van Vinh ; Baker, Stephen ; Delwart, Eric ; Thwaites, Guy ; Van Tan, Le
• Corporate Authors:
  for the VIZIONS Consortium1
• Description:
  Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections.
• Subjects:
  Central Nervous System Infections Cerebrospinal Fluid Enterovirus Enterovirus Infections Humans Metagenomics
• Source:
  Emerg Infect Dis. 27(1):205-213
• Pubmed ID:
  33350920
• Pubmed Central ID:
  PMC7774551
• Document Type:
  Journal Article
• Place as Subject:
  Vietnam
• Volume:
  27
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:aa3c52b28f19741a9c3ec1a6b63897b5c5278e55aa3eab255edc2186972b1046
• Download URL:
  https://stacks.cdc.gov/view/cdc/99983/cdc_99983_DS1.pdf
• File Type:
  [PDF - 1.78 MB ]