The Problem of Microbial Dark Matter in Neonatal Sepsis

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Sinnar, Shamim A. ; Schiff, Steven J.
• Description:
  Neonatal sepsis (NS) kills 750,000 infants every year. Effectively treating NS requires timely diagnosis and antimicrobial therapy matched to the causative pathogens, but most blood cultures for suspected NS do not recover a causative pathogen. We refer to these suspected but unidentified pathogens as microbial dark matter. Given these low culture recovery rates, many non-culture-based technologies are being explored to diagnose NS, including PCR, 16S amplicon sequencing, and whole metagenomic sequencing. However, few of these newer technologies are scalable or sustainable globally. To reduce worldwide deaths from NS, one possibility may be performing population-wide pathogen discovery. Because pathogen transmission patterns can vary across space and time, computational models can be built to predict the pathogens responsible for NS by region and season. This approach could help to optimally treat patients, decreasing deaths from NS and increasing antimicrobial stewardship until effective diagnostics that are scalable become available globally.
• Subjects:
  16S Amplicon Sequencing Antimicrobial Stewardship Bacteria Bloodborne Pathogens Cerebral Palsy Diagnosis DNA Sequence Analysis Escherichia Coli Group B Streptococcus Klebsiella Molecular Diagnosis Neonatal Sepsis Paenibacillus Thiaminolyticus Perspective Point Of Care Polymicrobial Infections RNA Sequence Analysis Schiff SJ. The Problem Of Microbial Dark Matter In Neonatal Sepsis. Emerg Infect Dis. 2020 Nov [date Cited]. Https://doi.org/10.3201/eid2611.200004 Staphylococcus Aureus Suggested Citation For This Article: Sinnar SA The Problem Of Microbial Dark Matter In Neonatal Sepsis Total Metagenomic Sequencing

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• Source:
  Emerg Infect Dis. 26(11):2543-2548
• Pubmed ID:
  33080169
• Pubmed Central ID:
  PMC7588532
• Document Type:
  Journal Article
• Volume:
  26
• Issue:
  11
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:c490690eb09e18651b4a6a3cd6727f16f88f88b9878faa7de56466da8af36bc3
• Download URL:
  https://stacks.cdc.gov/view/cdc/96899/cdc_96899_DS1.pdf
• File Type:
  [PDF - 520.34 KB ]