Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Paden, Clinton R.; Tao, Ying; Queen, Krista; Zhang, Jing; Li, Yan; Uehara, Anna; Tong, Suxiang

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Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2

Supporting Files Public Domain

July 01 2020
By Paden, Clinton R. ; Tao, Ying ; Queen, Krista ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Paden, Clinton R. ; Tao, Ying ; Queen, Krista ; Zhang, Jing ; Li, Yan ; Uehara, Anna ; Tong, Suxiang
Description:

We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Subjects:

Betacoronavirus Coronavirus Infections Covid-19 Multiplex Polymerase Chain Reaction Pandemics Pneumonia, Viral Rna, Viral Sequence Analysis, Rna Whole Genome Sequencing
Source:

Emerg Infect Dis. 26(10):2401-2405
Pubmed ID:

32610037
Pubmed Central ID:

PMC7510745
Document Type:

Journal Article
Volume:

26
Issue:

10
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:3e082892128843e2ac7878f31fcaedbc925856e425c2ce4770338ed2f51fce26
Download URL:

https://stacks.cdc.gov/view/cdc/94771/cdc_94771_DS1.pdf
File Type:

[PDF - 1.30 MB ]

File Language:

English

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