Middle East respiratory coronavirus (MERS-CoV) spike (S) protein vesicular stomatitis virus pseudoparticle neutralization assays offer a reliable alternative to the conventional neutralization assay in human seroepidemiological studies

Details

• Alternative Title:
  Access Microbiol
• Personal Author:
  Lester, Sandra ; Harcourt, Jennifer ; Whitt, Michael ; Al-Abdely, Hail M. ; Midgley, Claire M. ; Alkhamis, Abdulrahim M. ; Aziz Jokhdar, Hani A. ; Assiri, Abdullah M. ; Tamin, Azaibi ; Thornburg, Natalie
• Description:
  Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel zoonotic coronavirus that was identified in 2012. MERS-CoV infection in humans can result in an acute, severe respiratory disease and in some cases multi-organ failure; the global mortality rate is approximately 35 %. The MERS-CoV spike (S) protein is a major target for neutralizing antibodies in infected patients. The MERS-CoV microneutralization test (MNt) is the gold standard method for demonstrating prior infection. However, this method requires the use of live MERS-CoV in biosafety level 3 (BSL-3) containment. The present work describes the generation and validation of S protein-bearing vesicular stomatitis virus (VSV) pseudotype particles (VSV-MERS-CoV-S) in which the VSV glycoprotein G gene has been replaced by the luciferase reporter gene, followed by the establishment of a pseudoparticle-based neutralization test to detect MERS-CoV neutralizing antibodies under BSL-2 conditions. Using a panel of human sera from confirmed MERS-CoV patients, the VSV-MERS-CoV particle neutralization assay produced results that were highly comparable to those of the microneutralization test using live MERS-CoV. The results suggest that the VSV-MERS-CoV-S pseudotype neutralization assay offers a highly specific, sensitive and safer alternative method to detect MERS-CoV neutralizing antibodies in human sera.
• Subjects:
  Luciferase Microneutralization Test (MNt) Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Neutralizing Antibodies Pseudoparticle Research Article Vesicular Stomatitis Virus
• Source:
  Access Microbiol. 2019; 1(9)
• Pubmed ID:
  32974558
• Pubmed Central ID:
  PMC7472544
• Document Type:
  Journal Article
• Volume:
  1
• Issue:
  9
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6a84faf3fe4d87d40cd4550b677a8dd57fe6ce43483aba86e010941b1ffb7d1b
• Download URL:
  https://stacks.cdc.gov/view/cdc/94559/cdc_94559_DS1.pdf
• File Type:
  [PDF - 928.17 KB ]