Isolation, Sequence, Infectivity, and Replication Kinetics of Severe Acute Respiratory Syndrome Coronavirus 2

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Banerjee, Arinjay ; Nasir, Jalees A. ; Budylowski, Patrick ; Yip, Lily ; Aftanas, Patryk ; Christie, Natasha ; Ghalami, Ayoob ; Baid, Kaushal ; Raphenya, Amogelang R. ; Hirota, Jeremy A. ; Miller, Matthew S. ; McGeer, Allison J. ; Ostrowski, Mario ; Kozak, Robert A. ; McArthur, Andrew G. ; Mossman, Karen ; Mubareka, Samira
• Description:
  Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.
• Subjects:
  Betacoronavirus Coronavirus Infections Covid-19 DNA, Viral Genotype Humans Kinetics Leukocytes, Mononuclear Pandemics Pneumonia, Viral Polymorphism, Single Nucleotide Virus Replication Whole Genome Sequencing

  More +
• Source:
  Emerg Infect Dis. 26(9):2054-2063
• Pubmed ID:
  32558639
• Pubmed Central ID:
  PMC7454076
• Document Type:
  Journal Article
• Funding:
  CIHR/Canada
• Volume:
  26
• Issue:
  9
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:8ef83353c63a9e9883389eccab56f0862ac531de6485d015e72ee07af704ef6e
• Download URL:
  https://stacks.cdc.gov/view/cdc/93011/cdc_93011_DS1.pdf
• File Type:
  [PDF - 3.64 MB ]