Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Waggoner, Jesse J. ; Stittleburg, Victoria ; Pond, Renee ; Saklawi, Youssef ; Sahoo, Malaya K. ; Babiker, Ahmed ; Hussaini, Laila ; Kraft, Colleen S. ; Pinsky, Benjamin A. ; Anderson, Evan J. ; Rouphael, Nadine
• Description:
  Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
• Subjects:
  Betacoronavirus Coronavirus Infections Covid-19 Humans Multiplex Polymerase Chain Reaction Nasopharynx Real-Time Polymerase Chain Reaction
• Source:
  Emerg Infect Dis. 26(7):1633-1635
• Pubmed ID:
  32294051
• Pubmed Central ID:
  PMC7323516
• Document Type:
  Journal Article
• Genre:
  Letter
• Volume:
  26
• Issue:
  7
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:93b0c06013d4dc7a0fcd3cba1c7a06a8ce2d6c63475ce32d3490f4361f4a94df
• Download URL:
  https://stacks.cdc.gov/view/cdc/90211/cdc_90211_DS1.pdf
• File Type:
  [PDF - 275.26 KB ]