Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

Waggoner, Jesse J.; Stittleburg, Victoria; Pond, Renee; Saklawi, Youssef; Sahoo, Malaya K.; Babiker, Ahmed; Hussaini, Laila; Kraft, Colleen S.; Pinsky, Benjamin A.; Anderson, Evan J.; Rouphael, Nadine

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Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

June 21 2020
By Waggoner, Jesse J. ; Stittleburg, Victoria ; Pond, Renee ; ...
Source: Emerg Infect Dis. 26(7):1633-1635

[PDF-275.26 KB]

English

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Waggoner, Jesse J. ; Stittleburg, Victoria ; Pond, Renee ; Saklawi, Youssef ; Sahoo, Malaya K. ; ... More +

Waggoner, Jesse J. ; Stittleburg, Victoria ; Pond, Renee ; Saklawi, Youssef ; Sahoo, Malaya K. ; Babiker, Ahmed ; Hussaini, Laila ; Kraft, Colleen S. ; Pinsky, Benjamin A. ; Anderson, Evan J. ; Rouphael, Nadine Less -
Description:

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
Subjects:

[+]

Betacoronavirus Coronavirus Infections Covid-19 Humans Multiplex Polymerase Chain Reaction Nasopharynx Real-Time Polymerase Chain Reaction
Source:

Emerg Infect Dis. 26(7):1633-1635
Pubmed ID:

32294051
Pubmed Central ID:

PMC7323516
Document Type:

Journal Article
Genre:

Letter
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:93b0c06013d4dc7a0fcd3cba1c7a06a8ce2d6c63475ce32d3490f4361f4a94df
Download URL:

https://stacks.cdc.gov/view/cdc/90211/cdc_90211_DS1.pdf
File Type:

	20-1285-Techapp-s1.pdf	pdf
	20-1285.nxml	xml

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