Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Harcourt, Jennifer ; Tamin, Azaibi ; Lu, Xiaoyan ; Kamili, Shifaq ; Sakthivel, Senthil K. ; Murray, Janna ; Queen, Krista ; Tao, Ying ; Paden, Clinton R. ; Zhang, Jing ; Li, Yan ; Uehara, Anna ; Wang, Haibin ; Goldsmith, Cynthia ; Bullock, Hannah A. ; Wang, Lijuan ; Whitaker, Brett ; Lynch, Brian ; Gautam, Rashi ; Schindewolf, Craig ; Lokugamage, Kumari G. ; Scharton, Dionna ; Plante, Jessica A. ; Mirchandani, Divya ; Widen, Steven G. ; Narayanan, Krishna ; Makino, Shinji ; Ksiazek, Thomas G. ; Plante, Kenneth S. ; Weaver, Scott C. ; Lindstrom, Stephen ; Tong, Suxiang ; Menachery, Vineet D. ; Thornburg, Natalie J.
• Description:
  The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.
• Subjects:
  Animals Betacoronavirus Cell Line Chlorocebus Aethiops Coronavirus Infections Covid-19 Genome, Viral Humans Nasopharynx Oropharynx Pandemics Pneumonia, Viral Vero Cells Viral Tropism Virus Replication

  More +
• Source:
  Emerg Infect Dis. 26(6):1266-1273
• Pubmed ID:
  32160149
• Pubmed Central ID:
  PMC7258473
• Document Type:
  Journal Article
• Funding:
  U19 AI100625/AI/NIAID NIH HHS/United States ; R00 AG049092/AG/NIA NIH HHS/United States ; R24 AI120942/AI/NIAID NIH HHS/United States ; AI99107/NH/NIH HHS/United States ; AI114657/NH/NIH HHS/United States
• Place as Subject:
  Washington
• Location:
  North America
• Volume:
  26
• Issue:
  6
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:96d7f5b4212c4b6462f2a1237f9bea56f4767a035ffa2ac765b490cc5a256f11
• Download URL:
  https://stacks.cdc.gov/view/cdc/88906/cdc_88906_DS1.pdf
• File Type:
  [PDF - 2.78 MB ]