Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line

Kainulainen, Markus H.; Nichol, Stuart T.; Albariño, César G.; Spiropoulou, Christina F.

doi:10.1093/infdis/jix486

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Rapid Determination of Ebolavirus Infectivity in Clinical Samples Using a Novel Reporter Cell Line

12 12 2017
By Kainulainen, Markus H. ; Nichol, Stuart T. ; Albariño, César G. ; ...
http://dx.doi.org/10.1093/infdis/jix486
Source: J Infect Dis. 216(11):1380-1385

[PDF-682.28 KB]

English

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Details:

Alternative Title:

J Infect Dis
Personal Author:

Kainulainen, Markus H. ; Nichol, Stuart T. ; Albariño, César G. ; Spiropoulou, Christina F.

Kainulainen, Markus H. ; Nichol, Stuart T. ; Albariño, César G. ; Spiropoulou, Christina F. Less -
Description:

Modern ebolavirus diagnostics rely primarily on quantitative reverse transcription-polymerase chain reaction (qRT-PCR), a sensitive method to detect viral genetic material in the acute phase of the disease. However, qRT-PCR does not confirm presence of infectious virus, presenting limitations in patient and outbreak management. Attempts to isolate infectious virus rely on in vivo or basic cell culture approaches, which prohibit rapid results and screening. In this study, we present a novel reporter cell line capable of detecting live ebolaviruses. These cells permit sensitive, large-scale screening and titration of infectious virus in experimental and clinical samples, independent of ebolavirus species and variant.
Subjects:

[+]

Animals Cell Culture Techniques Cell Line Chlorocebus Aethiops Ebolavirus Genome, Viral Hemorrhagic Fever, Ebola Humans Molecular Diagnostic Techniques Real-Time Polymerase Chain Reaction RNA, Viral Sensitivity And Specificity Vero Cells
Keywords:

[+]

Ebola Infectivity Reporter Cell Line Virus Isolation
Source:

J Infect Dis. 216(11):1380-1385
Pubmed ID:

29029133
Pubmed Central ID:

PMC6050991
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHSUnited States/
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:badf61c4483c35ab9b31cda01a1f2f485da5d12b2d8012293612e3e3fa275b2e
Download URL:

https://stacks.cdc.gov/view/cdc/57419/cdc_57419_DS1.pdf
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