Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Becherer, Lisa ; Knauf, Sascha ; Marks, Michael ; Lueert, Simone ; Frischmann, Sieghard ; Borst, Nadine ; von Stetten, Felix ; Bieb, Sibauk ; Adu-Sarkodie, Yaw ; Asiedu, Kingsley ; Mitjà, Oriol ; Bakheit, Mohammed
• Description:
  Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar skin lesions, primarily Haemophilus ducreyi. We developed a novel molecular test to simultaneously detect T. pallidum and H. ducreyi based on mediator displacement LAMP. We validated the T. pallidum and H. ducreyi LAMP (TPHD-LAMP) by testing 293 clinical samples from patients with yaws-like lesions. Compared with quantitative PCR, the TPHD-LAMP demonstrated high sensitivity and specificity for T. pallidum (84.7% sensitivity, 95.7% specificity) and H. ducreyi (91.6% sensitivity, 84.8% specificity). This novel assay provided rapid molecular confirmation of T. pallidum and H. ducreyi DNA and might be suitable for use at the point of care. TPHD-LAMP could support yaws eradication by improving access to molecular diagnostic tests at the district hospital level.
• Subjects:
  Chancroid Child Female Haemophilus Ducreyi Humans Male Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques Sensitivity And Specificity Treponema Pallidum Yaws

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• Source:
  Emerg Infect Dis. 26(2):282-288
• Pubmed ID:
  31961303
• Pubmed Central ID:
  PMC6986840
• Document Type:
  Journal Article
• Place as Subject:
  Ghana ; Papua New Guinea
• Volume:
  26
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:2aa83634fd001ba2ed82a2bbeb882d92c77ae73eca6676aa252243fe58763b69
• Download URL:
  https://stacks.cdc.gov/view/cdc/84824/cdc_84824_DS1.pdf
• File Type:
  [PDF - 1.80 MB ]