High Pathogenicity of Nipah Virus from Pteropus lylei Fruit Bats, Cambodia

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Gaudino, Maria ; Aurine, Noémie ; Dumont, Claire ; Fouret, Julien ; Ferren, Marion ; Mathieu, Cyrille ; Reynard, Olivier ; Volchkov, Viktor E. ; Legras-Lachuer, Catherine ; Georges-Courbot, Marie-Claude ; Horvat, Branka
• Description:
  We conducted an in-depth characterization of the Nipah virus (NiV) isolate previously obtained from a Pteropus lylei bat in Cambodia in 2003 (CSUR381). We performed full-genome sequencing and phylogenetic analyses and confirmed CSUR381 is part of the NiV-Malaysia genotype. In vitro studies revealed similar cell permissiveness and replication of CSUR381 (compared with 2 other NiV isolates) in both bat and human cell lines. Sequence alignments indicated conservation of the ephrin-B2 and ephrin-B3 receptor binding sites, the glycosylation site on the G attachment protein, as well as the editing site in phosphoprotein, suggesting production of nonstructural proteins V and W, known to counteract the host innate immunity. In the hamster animal model, CSUR381 induced lethal infections. Altogether, these data suggest that the Cambodia bat-derived NiV isolate has high pathogenic potential and, thus, provide insight for further studies and better risk assessment for future NiV outbreaks in Southeast Asia.
• Subjects:
  Animals Genome, Viral Henipavirus Infections Humans Nipah Virus Phylogeny Real-Time Polymerase Chain Reaction Rna, Viral / Genetics Whole Genome Sequencing
• Source:
  Emerg Infect Dis. 26(1):104-113
• Pubmed ID:
  31855143
• Pubmed Central ID:
  PMC6924896
• Document Type:
  Journal Article
• Place as Subject:
  Cambodia
• Location:
  Asia
• Volume:
  26
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:adcbe3b6d45007e680016d6190f01488e3b00a64f6dbeb2b6f1426e3a54e8cb4
• Download URL:
  https://stacks.cdc.gov/view/cdc/83909/cdc_83909_DS1.pdf
• File Type:
  [PDF - 3.50 MB ]