Versatile cell ablation tools and their applications to study loss of cell functions

Details

• Alternative Title:
  Cell Mol Life Sci
• Personal Author:
  Liu, Fengming ; Dai, Shen ; Feng, Dechun ; Peng, Xiao ; Qin, Zhongnan ; Kearns, Alison C ; Huang, Wenfei ; Chen, Yong ; Ergün, Süleyman ; Wang, Hong ; Rappaport, Jay ; Bryda, Elizabeth C ; Chandrasekhar, Anand ; Aktas, Bertal ; Hu, Hongzhen ; Chang, Sulie L ; Gao, Bin ; Qin, Xuebin
• Description:
  Targeted cell ablation is a powerful approach for studying the role of specific cell populations in a variety of organotypic functions, including cell differentiation, and organ generation and regeneration. Emerging tools for permanently or conditionally ablating targeted cell populations and transiently inhibiting neuronal activities exhibit a diversity of application and utility. Each tool has distinct features, and none can be universally applied to study different cell types in various tissue compartments. Although these tools have been developed for over 30 years, they require additional improvement. Currently, there is no consensus on how to select the tools to answer the specific scientific questions of interest. Selecting the appropriate cell ablation technique to study the function of a targeted cell population is less straightforward than selecting the method to study a gene's functions. In this review, we discuss the features of the various tools for targeted cell ablation and provide recommendations for optimal application of specific approaches.
• Subjects:
  Animals Bacteriocins Clodronic Acid Diphtheria Toxin Humans MPTP Poisoning Neurons Optogenetics Receptors, G-Protein-Coupled Simplexvirus
• Keywords:
  Animal Models Bacterial Nitroreductase (NTR). Caspase Cell Interaction Chemogenetics Clodronate-liposome Diphtheria Toxin (DT) Human CD59 Intermedilysin (ILY) Thymidine Kinase (TK)
• Source:
  Cell Mol Life Sci. 76(23):4725-4743
• Pubmed ID:
  31359086
• Pubmed Central ID:
  PMC6858955
• Document Type:
  Journal Article
• Funding:
  T32 MH079785/MH/NIMH NIH HHSUnited States/ ; R21AA024984/AA/NIAAA NIH HHSUnited States/ ; R21 DA043448/DA/NIDA NIH HHSUnited States/ ; P40 OD011062/CD/ODCDC CDC HHSUnited States/ ; R01 DK104116/DK/NIDDK NIH HHSUnited States/ ; R21 AA024984/AA/NIAAA NIH HHSUnited States/ ; R01HL141132/HL/NHLBI NIH HHSUnited States/ ; P40 OD011062/OD/NIH HHSUnited States/ ; R01 DK113775/DK/NIDDK NIH HHSUnited States/ ; R01 HL141132/HL/NHLBI NIH HHSUnited States/ ; R01 AA027065/AA/NIAAA NIH HHSUnited States/ ; R01 HL117654/HL/NHLBI NIH HHSUnited States/ ; P51 OD011104/OD/NIH HHSUnited States/ ; R01 HL130233/HL/NHLBI NIH HHSUnited States/ ; R21OD024931/OD/ ; R21 OD024931/OD/NIH HHSUnited States/
• Volume:
  76
• Issue:
  23
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:254f99c320a18fab1b5cc4f1f2dddac65001ce4f017608bbdd8a2467db424832a80a8cc3d449f1b20107a06721c2f10ff71e6861dcfc01d86dbfb5ec504f818e
• Download URL:
  https://stacks.cdc.gov/view/cdc/83098/cdc_83098_DS1.pdf
• File Type:
  [PDF - 1.37 MB ]