Head-mounted central venous access during optical recordings and manipulations of neural activity in mice

Details

• Alternative Title:
  Nat Protoc
• Personal Author:
  Liu, Christine ; Freeman, Daniel J. ; Lammel, Stephan
• Description:
  Establishing reliable intravenous catheterization in mice with optical implants allows the combination of neural manipulations and recordings with rapid, time-locked delivery of pharmacological agents. Here we present a procedure for handmade jugular vein catheters designed for head-mounted intravenous access and provide surgical and postoperative guidance for improved survival and patency. A head-mounted vascular access point eliminates the need for a back-mounted button in animals already receiving neural implants, thereby reducing sites of implantation. This protocol, which is readily adoptable by experimenters with previous training and experience in mouse surgery, enables repeated fiber photometry recordings or optogenetic manipulation during drug delivery in adult mice that are awake and behaving, whether head fixed or freely moving. With practice, an experienced surgeon requires ~30 min to perform catheterization on each mouse. Altogether, these techniques facilitate the reliable and repeated delivery of pharmacological agents in mouse models while simultaneously recording at high temporal resolution and/or manipulating neural populations.
• Subjects:
  Animals Mice Optogenetics Prostheses And Implants
• Keywords:
  Alzheimer's Disease Alzheimer's Disease Sequencing Project Antiviral Agents Herpes Simplex Human Papillomavirus Torque Teno Viruses Whole Exome Sequencing Whole Genome Sequencing
• Source:
  Nat Protoc. 19(3):960-983
• Pubmed ID:
  38057625
• Pubmed Central ID:
  PMC10939862
• Document Type:
  Journal Article
• Funding:
  R01 DA042889/DA/NIDA NIH HHSUnited States/ ; K99 GM148823/GM/NIGMS NIH HHSUnited States/ ; RF1 MH123246/MH/NIMH NIH HHSUnited States/ ; R01 MH112721/MH/NIMH NIH HHSUnited States/ ; U01 IP000035/IP/NCIRD CDC HHSUnited States/
• Volume:
  19
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:1200e98d235eaa2ae55608f020a9323e28ede8f3a5975fa34746ae48a451b5c51508b5f6502d2be93f94e101c0fb8e78c222834e35a64dcdd21d84acd01426e6
• Download URL:
  https://stacks.cdc.gov/view/cdc/151717/cdc_151717_DS1.pdf
• File Type:
  [PDF - 1.89 MB ]