Details:

Alternative Title:
Nanoscale
Publisher's site:
http://dx.doi.org/10.1039/c5nr03411f
Personal Author:
Shah, Shreyas ; Liu, Jingjing ; Pasquale, Nicholas ; Lai, Jinping ; McGowan, Heather ; ... More +
Shah, Shreyas ; Liu, Jingjing ; Pasquale, Nicholas ; Lai, Jinping ; McGowan, Heather ; Pang, Zhiping P. ; Lee, Ki-Bum Less -
Description:
Nanotechnology-based approaches offer the chemical control required to develop precision tools suitable for applications in neuroscience. We report a novel approach employing hybrid upconversion nanomaterials, combined with the photoresponsive ion channel channelrhodopsin-2 (ChR2), to achieve near-infrared light (NIR)-mediated optogenetic control of neuronal activity. Current optogenetic methodologies rely on using visible light (e.g. 470 nm blue light), which tends to exhibit high scattering and low tissue penetration, to activate ChR2. In contrast, our approach enables the use of 980 nm NIR light, which addresses the short-comings of visible light as an excitation source. This was facilitated by embedding upconversion nanomaterials, which can convert NIR light to blue luminescence, into polymeric scaffolds. These hybrid nanomaterial scaffolds allowed for NIR-mediated neuronal stimulation, with comparable efficiency as that of 470 nm blue light. Our platform was optimized for NIR-mediated optogenetic control by balancing multiple physicochemical properties of the nanomaterial (e.g. size, morphology, structure, emission spectra, concentration), thus providing an early demonstration of rationally-designing nanomaterial-based strategies for advanced neural applications.
Subject:
[+]
Animals
Article
Cells, Cultured
Light
Mice
Nanostructures
Nanotechnology
Neurons
Optogenetics
Rhodopsin

Source:
Nanoscale. 7(40):16571-16577.
Pubmed ID:
26415758
Pubmed Central ID:
PMC4712042
Document Type:
Journal Article
Funding:
DP2 OD006462/OD/NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1R21NS085569-02/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; R21 DA035594/DA/NIDA NIH HHS/United States
DP2 OD006462/OD/NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1R21NS085569-02/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; R21 DA035594/DA/NIDA NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:de70c848d591e310576c03ee3433cfde31d10e5ad686849742678329d7b9703c
File Type:

Supporting Files

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