Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Okba, Nisreen M.A. ; Raj, V. Stalin ; Widjaja, Ivy ; GeurtsvanKessel, Corine H. ; de Bruin, Erwin ; Chandler, Felicity D. ; Park, Wan Beom ; Kim, Nam-Joong ; Farag, Elmoubasher A.B.A. ; Al-Hajri, Mohammed ; Bosch, Berend-Jan ; Oh, Myoung-don ; Koopmans, Marion P.G. ; Reusken, Chantal B.E.M. ; Haagmans, Bart L.
• Description:
  Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.
• Subjects:
  Antibodies, Neutralizing Antibodies, Viral Coronavirus Infections Enzyme-Linked Immunosorbent Assay Humans Immunity, Humoral Middle East Respiratory Syndrome Coronavirus Reverse Transcriptase Polymerase Chain Reaction Sensitivity And Specificity Severity Of Illness Index Time Factors

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• Source:
  Emerg Infect Dis. 25(10):1868-1877
• Pubmed ID:
  31423970
• Pubmed Central ID:
  PMC6759241
• Document Type:
  Journal Article
• Place as Subject:
  Netherlands ; Republic of Korea ; Qatar
• Location:
  Europe ; Asia ; Qatar
• Volume:
  25
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:a8ddde66415fa1d61c4164c6bb06f62baccc256cc58637d93f94ac91b3d2456d
• Download URL:
  https://stacks.cdc.gov/view/cdc/82948/cdc_82948_DS1.pdf
• File Type:
  [PDF - 1.96 MB ]