Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections

Okba, Nisreen M.A.; Raj, V. Stalin; Widjaja, Ivy; GeurtsvanKessel, Corine H.; de Bruin, Erwin; Chandler, Felicity D.; Park, Wan Beom; Kim, Nam-Joong; Farag, Elmoubasher A.B.A.; Al-Hajri, Mohammed; Bosch, Berend-Jan; Oh, Myoung-don; Koopmans, Marion P.G.; Reusken, Chantal B.E.M.; Haagmans, Bart L.

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections

Published Date:

October 17 2019

Source:

Emerg Infect Dis. 25(10):1868-1877

Language:

English

[PDF-1.96 MB]

Details:

Alternative Title:

Emerg Infect Dis

Personal Author:

Okba, Nisreen M.A. ; Raj, V. Stalin ; Widjaja, Ivy ; GeurtsvanKessel, Corine H. ; de Bruin, Erwin ; ... More ▼

Description:

Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein-based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43-positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.

Subject:

[+]

Pubmed ID:

31423970

Pubmed Central ID:

PMC6759241

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Main Document Checksum:

[+]

Supporting Files:

	19-0051-F5.gif	image/gif
	19-0051-F5.jpg	image/jpeg
	19-0051-Techapp-s1.pdf	application/pdf
	19-0051.nxml	text/xml
	19-0051-F1.gif	image/gif
	19-0051-F1.jpg	image/jpeg
	19-0051-F2.gif	image/gif
	19-0051-F2.jpg	image/jpeg
	19-0051-F3.gif	image/gif
	19-0051-F3.jpg	image/jpeg
	19-0051-F4.gif	image/gif
	19-0051-F4.jpg	image/jpeg

No Related Documents.

You May Also Like:

Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients

Personal Author:

Okba, Nisreen M.A.; Müller, Marcel A.; Li, Wentao; Wang, Chunyan; GeurtsvanKessel, Corine H.; Corman, Victor M.; Lamers, Mart M.; Sikkema, Reina S.; de Bruin, Erwin; Chandler, Felicity D.; Yazdanpanah, Yazdan; Le Hingrat, Quentin; Descamps, Diane; Houhou-Fidouh, Nadhira; Reusken, Chantal B.E.M.; Bosch, Berend-Jan; Drosten, Christian; Koopmans, Marion P.G.; Haagmans, Bart L.;

Published Date:

June 21 2020

Source:

Emerg Infect Dis. 26(7):1478-1488

Description:

A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Vali...

File Type:

[PDF - 3.14 MB]
Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

Personal Author:

Okba, Nisreen M.A.; Widjaja, Ivy; Li, Wentao; GeurtsvanKessel, Corine H.; Farag, Elmoubasher A.B.A.; Al-Hajri, Mohammed; Park, Wan Beom; Oh, Myoung-don; Reusken, Chantal B.E.M.; Koopmans, Marion P.G.; Bosch, Berend-Jan; Haagmans, Bart L.;

Published Date:

May 17 2020

Source:

Emerg Infect Dis. 26(5):1024-1027

Description:

We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assay...

File Type:

[PDF - 1.34 MB]
Antibody Response and Disease Severity in Healthcare Worker MERS Survivors

Personal Author:

Alshukairi, Abeer N.; Khalid, Imran; Ahmed, Waleed A.; Dada, Ashraf M.; Bayumi, Daniyah T.; Malic, Laut S.; Althawadi, Sahar; Ignacio, Kim; Alsalmi, Hanadi S.; Al-Abdely, Hail M.; Wali, Ghassan Y.; Qushmaq, Ismael A.; Alraddadi, Basem M.; Perlman, Stanley;

Published Date:

Jun 2016

Source:

Emerg Infect Dis. 22(6):1113-1115.

Description:

We studied antibody response in 9 healthcare workers in Jeddah, Saudi Arabia, who survived Middle East respiratory syndrome, by using serial ELISA and indirect immunofluorescence assay testing. Among patients who had experienced severe pneumonia, ant...

File Type:

[PDF - 464.27 KB]
Characteristics of Traveler with Middle East Respiratory Syndrome, China, 2015

Personal Author:

Da Guan, Wen; Mok, Chris Ka Pun; Chen, Zi Lin; Feng, Li Qiang; Li, Zheng Tu; Huang, Ji Cheng; Ke, Chang Wen; Deng, Xilong; Ling, Yun; Wu, Shi Guan; Niu, Xue Feng; Perera, Ranawaka A; Da Xu, Yuan; Zhao, Jincun; Zhang, Lin Qi; Li, Yi Min; Chen, Rong Chang; Peiris, Malik; Chen, Ling; Zhong, Nan Shan;

Published Date:

Dec 2015

Source:

Emerg Infect Dis. 21(12):2278-2280

File Type:

[PDF - 349.39 KB]
Middle East Respiratory Syndrome Coronavirus Seropositivity in Camel Handlers and Their Families, Pakistan

Personal Author:

Zheng, Jian; Hassan, Sohail; Alagaili, Abdulaziz N.; Alshukairi, Abeer N.; Amor, Nabil M.S.; Mukhtar, Nadia; Nazeer, Iqra Maleeha; Tahir, Zarfishan; Akhter, Nadeem; Perlman, Stanley; Yaqub, Tahir;

Published Date:

December 2019

Source:

Emerg Infect Dis. 25(12):2307-2309

Description:

A high percentage of camel handlers in Saudi Arabia are seropositive for Middle East respiratory syndrome coronavirus. We found that 12/100 camel handlers and their family members in Pakistan, a country with extensive camel MERS-CoV infection, were s...

File Type:

[PDF - 359.68 KB]