Isothermal Amplification Using a Chemical Heating Device for Point-of-Care Detection of HIV-1

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Curtis, Kelly A. ; Rudolph, Donna L. ; Nejad, Irene ; Singleton, Jered ; Beddoe, Andy ; Weigl, Bernhard ; LaBarre, Paul ; Owen, S. Michele
• Description:
  Background
  
  To date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagnosis or confirmation of infection status, as well as facilitating the diagnosis of acute infection, screening and evaluation of infants born to HIV-infected mothers. Isothermal amplification methods, such as reverse-transcription, loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for POC settings, since they are typically quicker, easier to perform, and allow for integration into low-tech, portable heating devices.
  
  Methodology/Significant Findings
  
  In this study, we evaluated the HIV-1 RT-LAMP assay using portable, non-instrumented nucleic acid amplification (NINA) heating devices that generate heat from the exothermic reaction of calcium oxide and water. The NINA heating devices exhibited stable temperatures throughout the amplification reaction and consistent amplification results between three separate devices and a thermalcycler. The performance of the NINA heaters was validated using whole blood specimens from HIV-1 infected patients.
  
  Conclusion
  
  The RT-LAMP isothermal amplification method used in conjunction with a chemical heating device provides a portable, rapid and robust NAAT platform that has the potential to facilitate HIV-1 testing in resource-limited settings and POC.
  
  
• Subjects:
  AIDS Serodiagnosis DNA, Viral DNA Primers Equipment Design Heating HIV-1 HIV Infections Humans Nucleic Acid Amplification Techniques Point-of-Care Systems RNA, Viral Temperature

  More +
• Source:
  PLoS One. 2012; 7(2).
• Document Type:
  Journal Article
• Volume:
  7
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:df88b6226e7f481fc428ec638b0e67634ac8ffa8a7c7c602b687d3eb999576fd
• Download URL:
  https://stacks.cdc.gov/view/cdc/8147/cdc_8147_DS1.pdf
• File Type:
  [PDF - 326.62 KB ]