Single-Use, Electricity-Free Amplification Device for Detection of HIV-1

Details

• Alternative Title:
  J Virol Methods
• Personal Author:
  Curtis, Kelly A. ; Rudolph, Donna L. ; Morrison, Daphne ; Guelig, Dylan ; Diesburg, Steven ; McAdams, David ; Burton, Robert A. ; LaBarre, Paul ; Owen, Michele
• Description:
  Early and accurate diagnosis of HIV is key for the reduction of transmission and initiation of patient care. The availability of a rapid nucleic acid test (NAT) for use at the point-of-care (POC) will fill a gap in HIV diagnostics, improving the diagnosis of acute infection and HIV in infants born to infected mothers. In this study, we evaluated the performance of non-instrumented nucleic acid amplification, single-use disposable (NINA-SUD) devices for the detection of HIV-1 in whole blood using reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) with lyophilized reagents. The NINA-SUD heating device harnesses the heat from an exothermic chemical reaction initiated by the addition of saline to magnesium iron powder. Reproducibility was demonstrated between NINA-SUD units and comparable, if not superior, performance for detecting clinical specimens was observed as compared to the thermal cycler. The stability of the lyophilized HIV-1 RT-LAMP reagents was also demonstrated following storage at -20, 4, 25, and 30°C for up to one month. The single-use, disposable NAT minimizes hands-on time and has the potential to facilitate HIV-1 testing in resource-limited settings or at the POC.
• Subjects:
  Article Diagnosis Electricity HIV-1 HIV Infections Hot Temperature Humans Nucleic Acid Amplification Nucleic Acid Amplification Techniques Point-of-Care Point-of-Care Systems Reproducibility Of Results RNA RNA, Viral Sensitivity And Specificity

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• Source:
  J Virol Methods. 237:132-137
• Pubmed ID:
  27616198
• Pubmed Central ID:
  PMC5056841
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/ ; P30 AI027757/AI/NIAID NIH HHSUnited States/ ; R01 EB012641/EB/NIBIB NIH HHSUnited States/
• Volume:
  237
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:fddddf65e7bc1f93f961196c24fc185ffa34eb5a0fe07bc66ec8e67c03170be3
• Download URL:
  https://stacks.cdc.gov/view/cdc/42184/cdc_42184_DS1.pdf
• File Type:
  [PDF - 470.96 KB ]