Details:

Alternative Title:
J Virol Methods
Publisher's site:
http://dx.doi.org/10.1016/j.jviromet.2016.09.007
Personal Author:
Curtis, Kelly A. ; Rudolph, Donna L. ; Morrison, Daphne ; Guelig, Dylan ; Diesburg, Steven ; ... More +
Curtis, Kelly A. ; Rudolph, Donna L. ; Morrison, Daphne ; Guelig, Dylan ; Diesburg, Steven ; McAdams, David ; Burton, Robert A. ; LaBarre, Paul ; Owen, Michele Less -
Description:
Early and accurate diagnosis of HIV is key for the reduction of transmission and initiation of patient care. The availability of a rapid nucleic acid test (NAT) for use at the point-of-care (POC) will fill a gap in HIV diagnostics, improving the diagnosis of acute infection and HIV in infants born to infected mothers. In this study, we evaluated the performance of non-instrumented nucleic acid amplification, single-use disposable (NINA-SUD) devices for the detection of HIV-1 in whole blood using reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) with lyophilized reagents. The NINA-SUD heating device harnesses the heat from an exothermic chemical reaction initiated by the addition of saline to magnesium iron powder. Reproducibility was demonstrated between NINA-SUD units and comparable, if not superior, performance for detecting clinical specimens was observed as compared to the thermal cycler. The stability of the lyophilized HIV-1 RT-LAMP reagents was also demonstrated following storage at -20, 4, 25, and 30°C for up to one month. The single-use, disposable NAT minimizes hands-on time and has the potential to facilitate HIV-1 testing in resource-limited settings or at the POC.
Subject:
[+]
Article
Diagnosis
Electricity
HIV-1
HIV Infections
Hot Temperature
Humans
Nucleic Acid Amplification
Nucleic Acid Amplification Techniques
Point-of-Care
Point-of-Care Systems
Reproducibility Of Results
RNA
RNA, Viral
Sensitivity And Specificity

Source:
J Virol Methods. 237:132-137.
Pubmed ID:
27616198
Pubmed Central ID:
PMC5056841
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States ; P30 AI027757/AI/NIAID NIH HHS/United States ; R01 EB012641/EB/NIBIB NIH HHS/United States
CC999999/Intramural CDC HHS/United States ; P30 AI027757/AI/NIAID NIH HHS/United States ; R01 EB012641/EB/NIBIB NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fddddf65e7bc1f93f961196c24fc185ffa34eb5a0fe07bc66ec8e67c03170be3
File Type:

Supporting Files

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