Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0126609
Personal Author:
Rudolph, Donna L. ; Sullivan, Vickie ; Owen, S. Michele ; Curtis, Kelly A. ;
Rudolph, Donna L. ; Sullivan, Vickie ; Owen, S. Michele ; Curtis, Kelly A. ; Less -
Description:
A rapid, cost-effective diagnostic test for the detection of acute HIV-1 infection is highly desired. Isothermal amplification techniques, such as reverse-transcription loop-mediated isothermal amplification (RT-LAMP), exhibit characteristics that are ideal for the development of a rapid nucleic acid amplification test (NAAT) because they are quick, easy to perform and do not require complex, dedicated equipment and laboratory space. In this study, we assessed the ability of the HIV-1 RT-LAMP assay to detect acute HIV infection as compared to a representative rapid antibody test and several FDA-approved laboratory-based assays. The HIV-1 RT-LAMP assay detected seroconverting individuals one to three weeks earlier than a rapid HIV antibody test and up to two weeks earlier than a lab-based antigen/antibody (Ag/Ab) combo enzyme immunoassay (EIA). RT-LAMP was not as sensitive as a lab-based qualitative RNA assay, which could be attributed to the significantly smaller nucleic acid input volume. To our knowledge, this is the first demonstration of detecting acute HIV infection using the RT-LAMP assay. The availability of a rapid NAAT, such as the HIV-1 RT-LAMP assay, at the point of care (POC) or in laboratories that do not have access to large platform NAAT could increase the percentage of individuals who receive an acute HIV infection status or confirmation of their HIV status, while immediately linking them to counseling and medical care. In addition, early knowledge of HIV status could lead to reduced high-risk behavior at a time when individuals are at a higher risk for transmitting the virus.
Subject:
[+]
Biological Assay
Cell Line
DNA Primers
Early Diagnosis
HIV-1
HIV Infections
Humans
Immune Sera
Lymphocytes
Monocytes
Nucleic Acid Amplification Techniques
Point-of-Care Systems
Research Article
Reverse Transcription
RNA, Viral
Sensitivity And Specificity
Ultraviolet Rays

Source:
PLoS One. 10(5). ;
Pubmed ID:
25993381
Pubmed Central ID:
PMC4439053
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:ebc192bb15445a777b38996d1e06ec35c80b19802167de6f3847f50df12f15e9
File Type:

Supporting Files

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