PRECONDITIONING IN THE RHESUS MACAQUE INDUCES A PROTEOMIC SIGNATURE FOLLOWING CEREBRAL ISCHEMIA THAT IS ASSOCIATED WITH NEUROPROTECTION

Details

• Alternative Title:
  Transl Stroke Res
• Personal Author:
  Stevens, Susan L ; Liu, Tao ; Bahjat, Frances Rena ; Petyuk, Vladislav A. ; Schepmoes, Athena A. ; Sontag, Ryan L. ; Gritsenko, Marina A. ; Wu, Chaochao ; Wang, Sheng ; Shukla, Anil K. ; Jacobs, Jon M. ; Smith, Richard D. ; Rodland, Karin D. ; West, G. Alexander ; Kohama, Steven G. ; Glynn, Christine ; Stenzel-Poore, Mary P.
• Description:
  Each year, thousands of patients are at risk of cerebral ischemic injury, due to iatrogenic responses to surgical procedures. Prophylactic treatment of these patients as standard care could minimize potential neurological complications. We have shown that protection of brain tissue, in a non-human primate model of cerebral ischemic injury, is possible through pharmacological preconditioning using the immune activator D192935. We postulate that preconditioning with D192935 results in neuroprotective reprogramming that is evident in the brain following experimentally induced cerebral ischemia. We performed quantitative proteomic analysis of cerebral spinal fluid (CSF) collected post-stroke from our previously published efficacy study to determine whether CSF protein profiles correlated with induced protection. Four groups of animals were examined: naïve animals (no treatment or stroke); animals treated with vehicle prior to stroke; D192935 treated and stroked animals, further delineated into two groups, ones that were protected (small infarcts) and those that were not protected (large infarcts). We found that distinct protein clusters defined the protected and non-protected animal groups, with a 16-member cluster of proteins induced exclusively in D192935 protected animals. Seventy percent of the proteins induced in the protected animals have functions that would enhance neuroprotection and tissue repair, including several members associated with M2 macrophages, a macrophage phenotype shown to contribute to neuroprotection and repair during ischemic injury. These studies highlight the translational importance of CSF biomarkers in defining mechanism and monitoring responses to treatment in development of stroke therapeutics.
• Subjects:
  Article Cerebral Spinal Fluid Neuroprotection Non-human Primates Proteomics Stroke
• Source:
  Transl Stroke Res. 10(4):440-448
• Pubmed ID:
  30341719
• Pubmed Central ID:
  PMC6474839
• Document Type:
  Journal Article
• Funding:
  P41 GM103493/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P51 OD011092/ODCDC CDC HHS/Office of the Director/United States ; U01 NS064953/NINDS NIH HHS/National Institute of Neurological Disorders and Stroke/United States
• Volume:
  10
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ea58d0349252a77bdb29135ee4ba8e559db2f57c4aaffd3d0afd795a5661ac78
• Download URL:
  https://stacks.cdc.gov/view/cdc/80493/cdc_80493_DS1.pdf
• File Type:
  [PDF - 1.23 MB ]