Defining blood hematology reference values in female pig-tailed macaques (Macaca nemestrina) using the Isolation Forest algorithm

Details

• Alternative Title:
  J Med Primatol
• Personal Author:
  Kim, Daniel ; Derton, Abigail ; Khalil, George ; Pan, Yi ; Bachman, Shanon ; Kelley, Kristen ; Garcίa-Lerma, Gerardo ; Dobard, Charles W. ; Daly, Michele B.
• Description:
  Background:
  
  Pig-tailed macaques (PTMs) are commonly used as preclinical models to assess antiretroviral drugs for HIV prevention research. Drug toxicities and disease pathologies are often preceded by changes in blood hematology. To better assess the safety profile of pharmaceuticals, we defined normal ranges of hematological values in PTMs using an Isolation Forest (iForest) algorithm.
  
  Methods:
  
  Eighteen female PTMs were evaluated. Blood was collected 1–24 times per animal for a total of 159 samples. Complete blood counts were performed, and iForest was used to analyze the hematology data to detect outliers.
  
  Results:
  
  Median, IQR, and ranges were calculated for 13 hematology parameters. From all samples, 22 outliers were detected. These outliers were excluded from the reference index.
  
  Conclusions:
  
  Using iForest, we defined a normal range for hematology parameters in female PTMs. This reference index can be a valuable tool for future studies evaluating drug toxicities in PTMs.
  
  
• Subjects:
  Algorithms Animals Female Hematologic Tests Macaca Nemestrina Reference Values
• Keywords:
  Complete Blood Counts Drug Toxicities Non-human Primates
• Source:
  J Med Primatol. 53(4):e12723
• Pubmed ID:
  38978165
• Pubmed Central ID:
  PMC11313183
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  53
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:e58bd0ce818c05d8971a1dae8cdbb05a3418bee324389ed6702bab5c6a8777708431d91450b95acb09b280675e0972e57bad53d259f89b8e0e5981748a7a85ef
• Download URL:
  https://stacks.cdc.gov/view/cdc/159789/cdc_159789_DS1.pdf
• File Type:
  [PDF - 569.51 KB ]