Quantitative Proteomic Analysis Reveals Caffeine-Perturbed Proteomic Profiles in Normal Bladder Epithelial Cells

Details

• Alternative Title:
  Proteomics
• Personal Author:
  Shahid, Muhammad ; Kim, Minhyung ; Yeon, Austin ; Andres, Allen M. ; You, Sungyong ; Kim, Jayoung
• Description:
  Lower urinary tract symptoms (LUTSs) are highly prevalent among the elderly and negatively impact quality of life. Since caffeinated beverages are enjoyed worldwide and the relationship between LUTS and caffeine is still not fully understood, it would be of particular interest to examine the underlying mechanisms that drive caffeine's influence on LUTS development and progression. The aim of this study is to characterize the effects of caffeine on hTert-immortalized normal bladder epithelial cells by investigating whether exposure to caffeine can cause potential changes in the bladder proteome and/or biological pathways. In labeled LC-MS/MS proteomic analysis, 57 proteins are found as being differentially expressed in caffeine-treated bladder epithelial cells, compared to controls; this included 32 upregulated and 25 downregulated proteins. Further functional gene enrichment analysis reveals that caffeine affects major biological pathways, including those for "muscle contraction" and "chromatin assembly." These findings provide new scientific insights that may be useful in future studies investigating the role of caffeine in bladder dysfunctions.
• Subjects:
  Article Biological Network Bladder Epithelial Cells Caffeine Cells, Cultured Central Nervous System Stimulants Chromatography, Liquid Epithelial Cells Global Proteome Humans Lower Urinary Tract Symptoms Proteome Tandem Mass Spectrometry Urinary Bladder

  More +
• Source:
  Proteomics. 18(20):e1800190
• Pubmed ID:
  30232827
• Pubmed Central ID:
  PMC6493332
• Document Type:
  Journal Article
• Funding:
  U01DP006079/ACL HHS/United States ; UL1 TR000124/TR/NCATS NIH HHS/United States ; U24 DK097154/DK/NIDDK NIH HHS/United States ; 1R01DK100974/DK/NIDDK NIH HHS/United States ; 1U01DK103260/DK/NIDDK NIH HHS/United States ; NIH NCATS UCLA CTSI UL1TR000124/TR/NCATS NIH HHS/United States ; U01 DP006079/DP/NCCDPHP CDC HHS/United States ; R01 DK100974/DK/NIDDK NIH HHS/United States ; U01 DK103260/DK/NIDDK NIH HHS/United States ; 1U01DP006079/DP/NCCDPHP CDC HHS/United States
• Volume:
  18
• Issue:
  20
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:3042018aaf45b392f5c166c8687dfd86d6049a8296a265ff93038ede4e62ce6f
• Download URL:
  https://stacks.cdc.gov/view/cdc/78315/cdc_78315_DS1.pdf
• File Type:
  [PDF - 1.49 MB ]