Candida parapsilosis Characterization in an Outbreak Setting

Kuhn, Duncan M.; Mukherjee, Pranab K.; Clark, Thomas A.; Pujol, Claude; Chandra, Jyotsna; Hajjeh, Rana A.; Warnock, David W.; Soll, David R.; Ghannoum, Mahmoud A.

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Candida parapsilosis Characterization in an Outbreak Setting

Supporting Files Public Domain

Jun 2004
By Kuhn, Duncan M. ; Mukherjee, Pranab K. ; Clark, Thomas A. ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Kuhn, Duncan M. ; Mukherjee, Pranab K. ; Clark, Thomas A. ; Pujol, Claude ; Chandra, Jyotsna ; Hajjeh, Rana A. ; Warnock, David W. ; Soll, David R. ; Ghannoum, Mahmoud A.
Description:

Candida parapsilosis is an important non-albicans species which infects hospitalized patients. No studies have correlated outbreak infections of C. parapsilosis with multiple virulence factors. We used DNA fingerprinting to determine genetic variability among isolates from a C. parapsilosis outbreak and from our clinical database. We compared phenotypic markers of pathogenesis, including adherence, biofilm formation, and protein secretion (secretory aspartic protease [SAP] and phospholipase). Adherence was measured as colony counts on silicone elastomer disks immersed in agar. Biofilms formed on disks were quantified by dry weight. SAP expression was measured by hydrolysis of bovine albumin; a colorimetric assay was used to quantitate phospholipase. DNA fingerprinting indicated that the outbreak isolates were clonal and genetically distinct from our database. Biofilm expression by the outbreak clone was greater than that of sporadic isolates (p < or =0.0005). Adherence and protein secretion did not correlate with strain pathogenicity. These results suggest that biofilm production plays a role in C. parapsilosis outbreaks.
Subjects:

Aspartic Acid Endopeptidases Bacterial Adhesion Biofilms Blotting, Southern Candida Candidiasis Cluster Analysis Disease Outbreaks DNA, Fungal DNA Fingerprinting Humans Phospholipases Phylogeny Research
Source:

Emerg Infect Dis. 10(6):1074-1081.
Document Type:

Journal Article
Place as Subject:

Mississippi
Location:

North America
Volume:

10
Issue:

6
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:7e5259e91f5a517979950a4ec4552fa1023ae28c29cda657aa0c7774bf3c509f
Download URL:

https://stacks.cdc.gov/view/cdc/7765/cdc_7765_DS1.pdf
File Type:

[PDF - 378.04 KB ]

File Language:

English

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