Simplified Model to Survey Tuberculosis Transmission in Countries Without Systematic Molecular Epidemiology Programs

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Domínguez, Juan ; Acosta, Fermín ; Pérez-Lago, Laura ; Sambrano, Dilcia ; Batista, Victoria ; De La Guardia, Carolina ; Abascal, Estefanía ; Chiner-Oms, Álvaro ; Comas, Iñaki ; González, Prudencio ; Bravo, Jaime ; Del Cid, Pedro ; Rosas, Samantha ; Muñoz, Patricia ; Goodridge, Amador ; García de Viedma, Darío
• Description:
  Systematic molecular/genomic epidemiology studies for tuberculosis surveillance cannot be implemented in many countries. We selected Panama as a model for an alternative strategy. Mycobacterial interspersed repetitive unit-variable-number tandem-repeat (MIRU-VNTR) analysis revealed a high proportion (50%) of Mycobacterium tuberculosis isolates included in 6 clusters (A-F) in 2 provinces (Panama and Colon). Cluster A corresponded to the Beijing sublineage. Whole-genome sequencing (WGS) differentiated clusters due to active recent transmission, with low single-nucleotide polymorphism-based diversity (cluster C), from clusters involving long-term prevalent strains with higher diversity (clusters A, B). Prospective application in Panama of 3 tailored strain-specific PCRs targeting marker single-nucleotide polymorphisms identified from WGS data revealed that 31.4% of incident cases involved strains A-C and that the Beijing strain was highly represented and restricted mainly to Colon. Rational integration of MIRU-VNTR, WGS, and tailored strain-specific PCRs could be a new model for tuberculosis surveillance in countries without molecular/genomic epidemiology programs.
• Subjects:
  Bacteria Epidemiology Model Research Simplified Model To Survey Tuberculosis Transmission In Countries Without Systematic Molecular Epidemiology Programs Strain-specific PCR Surveillance TB Transmission Tuberculosis Tuberculosis And Other Mycobacteria Whole-genome Sequencing

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• Source:
  Emerg Infect Dis. 25(3):507-514
• Pubmed ID:
  30789134
• Pubmed Central ID:
  PMC6390753
• Document Type:
  Journal Article
• Place as Subject:
  Panama
• Location:
  Central America
• Volume:
  25
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:804f6e8b799cf05dca257052b86976329e59e6ff863135e6099695371258f4c3
• Download URL:
  https://stacks.cdc.gov/view/cdc/76664/cdc_76664_DS1.pdf
• File Type:
  [PDF - 924.46 KB ]