Vitamin D Supplementation Protects Against Bone Loss Following Inhalant Organic Dust and Lipopolysaccharide Exposures in Mice

Details

• Alternative Title:
  Immunol Res
• Personal Author:
  Dusad, Anand ; Thiele, Geoffrey M. ; Klassen, Lynell W. ; Wang, Dong ; Duryee, Michael J. ; Mikuls, Ted R. ; Staab, Elizabeth B. ; Wyatt, Todd A. ; West, William W. ; Reynolds, Stephen J. ; Romberger, Debra J. ; Poole, Jill A.
• Description:
  Systemic bone loss is associated with airway inflammatory diseases; yet, strategies to halt disease progression from inhalant exposures are not clear. Vitamin D might be a potentially protective approach against noxious respirable environmental exposures. We sought to determine whether vitamin D supplementation represents a viable lung- and bone-protective strategy following repetitive inhalant treatments with organic dust extract (ODE) or lipopolysaccharide (LPS) in mice. C57BL/5 mice were maintained on diets with low (1 IU/D/g) or high (10 IU/D/g) vitamin D for 5 weeks and treated with ODE from swine confinement facilities, LPS, or saline daily for 3 weeks per established intranasal inhalation protocol. Lungs, hind limbs, and sera were harvested for experimental outcomes. Serum 25-hydroxyvitamin D levels were tenfold different between low and high vitamin D treatment groups with no differences between inhalant agents and saline treatments. Serum calcium levels were not affected. There was no difference in the magnitude of ODE- or LPS-induced inflammatory cell influx or lung histopathology between high and low vitamin D treatment groups. However, high vitamin D treatment reversed the loss of bone mineral density, bone volume, and bone micro-architecture deterioration induced by ODE or LPS as determined by micro-CT analysis. Bone-resorbing osteoclasts were also reduced by high vitamin D treatment. In the low vitamin D treatment groups, ODE induced the greatest degree of airway inflammatory consequences, and LPS induced the greatest degree of bone loss. Collectively, high-concentration vitamin D was protective against systemic bone loss, but not airway inflammation, resulting from ODE- or LPS-induced airway injury.
• Subjects:
  Administration, Intranasal Animal Models Animals Article Bone Interactors-other Bone Resorption Bronchoalveolar Lavage Fluid Calcium Cytokines Dietary Supplements Disease Disorder Dust Femur Housing, Animal Lipopolysaccharides Lung Male Mice, Inbred C57BL Osteoporosis Pneumonia Preclinical Radiography Swine Systems Biology Tibia Vitamin D

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• Source:
  Immunol Res. 62(1):46-59
• Pubmed ID:
  25759026
• Pubmed Central ID:
  PMC4426061
• Document Type:
  Journal Article
• Funding:
  R01 AR062680/AR/NIAMS NIH HHS/United States ; R01 ES019325/ES/NIEHS NIH HHS/United States ; U54 OH010162/OH/NIOSH CDC HHS/United States
• Volume:
  62
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c7ed703fc220e74cfbad4b719637291717d5da3ef1b72a8c3dbcef5aea1400e6
• Download URL:
  https://stacks.cdc.gov/view/cdc/75899/cdc_75899_DS1.pdf
• File Type:
  [PDF - 3.23 MB ]