Vitamin D Supplementation Protects Against Bone Loss Following Inhalant Organic Dust and Lipopolysaccharide Exposures in Mice

Details

• Personal Author:
  Duryee MJ ; Dusad A ; Klassen LW ; Mikuls TR ; Poole JA ; Reynolds SJ ; Romberger DJ ; Staab EB ; Thiele GM ; Wang D ; West WW ; Wyatt TA
• Description:
  Systemic bone loss is associated with airway inflammatory diseases; yet, strategies to halt disease progression from inhalant exposures are not clear. Vitamin D might be a potentially protective approach against noxious respirable environmental exposures. We sought to determine whether vitamin D supplementation represents a viable lung and bone protective strategy following repetitive inhalant treatments with organic dust extract (ODE) or lipopolysaccharide (LPS) in mice. C57BL/5 mice were maintained on diets with low (1 IU/D/g) or high (10 IU/D/g) vitamin D for 5 weeks, and treated with ODE from swine confinement facilities, LPS, or saline daily for 3 weeks per established intranasal inhalation protocol. Lungs, hind limbs, and sera were harvested for experimental outcomes. Serum 25-hydroxy vitamin D levels were 10-fold different between low/high vitamin D treatment groups with no differences between inhalant agents/saline treatments. Serum calcium levels were not affected. There was no difference in the magnitude of ODE- or LPS-induced inflammatory cell influx or lung histopathology between high/low vitamin D treatment groups. However, high vitamin D treatment reversed the loss of bone mineral density, bone volume, and bone microarchitecture deterioration induced by ODE or LPS as determined by micro-CT analysis. Bone-resorbing osteoclasts were also reduced by high vitamin D treatment. In the low vitamin D treatment groups, ODE induced the greatest degree of airway inflammatory consequences, and LPS induced the greatest degree of bone loss. Collectively, high concentration vitamin D was protective against systemic bone loss, but not airway inflammation, resulting from ODE- or LPS-induced airway injury. [Description provided by NIOSH]
• Subjects:
  Animals Dust Lipids Lung Occupational Exposure Occupational Health Respiratory System Safety Steroids
• Keywords:
  Animal Studies Author Keywords: Animal Models Bone Interactors-other Bone Structure Chronic Exposure Disease Disorder Hormone Activity Inhalation Studies Lipopolysaccharide Lung Irritants Lung Tissue Organic Dusts Osteoporosis Preclinical Systems Biology Vitamins

  More +
• ISSN:
  0257-277X
• Document Type:
  Text
• Funding:
  Cooperative Agreement ; Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Iowa ; Nebraska ; OSHA Region 7 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  46-59
• Volume:
  62
• Issue:
  1
• NIOSHTIC Number:
  nn:20052053
• Citation:
  Immunol Res 2015 May; 62(1):46-59
• Contact Point Address:
  Jill A. Poole, Pulmonary, Critical Care, Sleep & Allergy Division, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, USA
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Colorado State University - Ft. Collins
• Peer Reviewed:
  True
• Start Date:
  20030915
• Source Full Name:
  Immunologic Research
• End Date:
  20270914
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:324f578a0d890c6615f62906696fd950cc69c45012025fea33594e081c8727a4657d9bd9171c422a865833c08fb722312dc78002095810d6287812cbd2ff2712
• Download URL:
  https://stacks.cdc.gov/view/cdc/211660/cdc_211660_DS1.pdf
• File Type:
  [PDF - 2.35 MB ]