Details:

Alternative Title:
Wellcome Open Res
Personal Author:
Jeffries, Claire L. ; Lawrence, Gena G. ; Golovko, George ; Kristan, Mojca ; Orsborne, James ; ... More +
Jeffries, Claire L. ; Lawrence, Gena G. ; Golovko, George ; Kristan, Mojca ; Orsborne, James ; Spence, Kirstin ; Hurn, Eliot ; Bandibabone, Janvier ; Tantely, Luciano M. ; Raharimalala, Fara N. ; Keita, Kalil ; Camara, Denka ; Barry, Yaya ; Wat’senga, Francis ; Manzambi, Emile Z. ; Afrane, Yaw A. ; Mohammed, Abdul R. ; Abeku, Tarekegn A. ; Hedge, Shivanand ; Khanipov, Kamil ; Pimenova, Maria ; Fofanov, Yuriy ; Boyer, Sebastien ; Irish, Seth R. ; Hughes, Grant L. ; Walker, Thomas Less -
Description:
Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing endosymbiotic bacterium Asaia.

Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis of samples was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene.

Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species ‘A’, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species ‘A’. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species ‘A’, but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were also shown to be variable and location dependent

Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.


Subjects:
[+]
Aged Anesthesia, Epidural Anesthesia, General Antibodies, Monoclonal Female Hip Prosthesis Humans Leukocyte Count Male Random Allocation T-Lymphocytes T-Lymphocytes, Helper-Inducer T-Lymphocytes, Regulatory Thymidine
Source:
Wellcome Open Res. 2018; 3.
Pubmed Central ID:
PMC6234743
Document Type:
Journal Article
Place as Subject:
Africa South of the Sahara
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:2d40c47e613acb66f3c4745fdedfb3c96949f1059372ddd738135263d4952546
Download URL:
https://stacks.cdc.gov/view/cdc/60844/cdc_60844_DS1.pdf
File Type:

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