Details:

Alternative Title:
J Food Prot
Personal Author:
PATEL, ISHA R.; GANGIREDLA, JAYANTHI; LACHER, DAVID W.; MAMMEL, MARK K.; BAGI, LORI; ... More +
PATEL, ISHA R.; GANGIREDLA, JAYANTHI; LACHER, DAVID W.; MAMMEL, MARK K.; BAGI, LORI; BARANZONI, GIAN MARCO; FRATAMICO, PINA M.; ROBERTS, ELIZABETH L.; DebROY, CHITRITA; LINDSEY, REBECCA L.; STONEBURG, DEVON V.; MARTIN, HALEY; SMITH, PEYTON; STROCKBINE, NANCY A.; ELKINS, CHRISTOPHER A.; SCHEUTZ, FLEMMING; FENG, PETER C. H.; Less -
Description:
The U.S. Food and Drug Administration Escherichia coli Identification (FDA-ECID) microarray provides rapid molecular characterization of E. coli. The effectiveness of the FDA-ECID for characterizing Shiga toxin-producing E. coli (STEC) was evaluated by three federal laboratories and one reference laboratory with a panel of 54 reference E. coli strains from the External Quality Assurance program. Strains were tested by FDA-ECID for molecular serotyping (O and H antigens), Shiga toxin subtyping, and the presence of the ehxA and eae genes for enterohemolysin and intimin, respectively. The FDA-ECID O typing was 96% reproducible among the four laboratories and 94% accurate compared with the reference External Quality Assurance data. Discrepancies were due to the absence of O41 target loci on the array and to two pairs of O types with identical target sequences. H typing was 96% reproducible and 100% accurate, with discrepancies due to two strains from one laboratory that were identified as mixed by FDA-ECID. Shiga toxin (Stx) type 1 subtyping was 100% reproducible and accurate, and Stx2 subtyping was 100% reproducible but only 64% accurate. FDA-ECID identified most Stx2 subtypes but had difficulty distinguishing among stx|, stx|, and stx| genes because of close similarities of these sequences. FDA-ECID was 100% effective for detecting ehxA and eae and accurately subtyped the eae alleles. This interlaboratory study revealed that FDA-ECID for STEC characterization was highly reproducible for molecular serotyping, stx and eae subtyping, and ehxA detection. However, the array was less useful for distinguishing among the highly homologous O antigen genes and the stx|, stx|, and stx| subtypes.
Subject:
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Escherichia Coli Proteins
Food Microbiology
Humans
Serotyping
Shiga-Toxigenic Escherichia Coli
Shiga Toxin
Shiga Toxin 1
Virulence

Source:
J Food Prot. 81(8):1275-1282;
Pubmed ID:
29985068
Pubmed Central ID:
PMC6193752
Document Type:
Journal Article;
Funding:
CC999999/Intramural CDC HHS/United States;
Name as Subject:
United States. Food and Drug Administration.;
Place as Subject:
United States;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:181c4d8859800f2331df652c347e8813947715cd3cc4d22a673815be39c75dc3
File Type:

Supporting Files

• nihms-991738.nxml

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