Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Costantini, Veronica ; Morantz, Esther K. ; Browne, Hannah ; Ettayebi, Khalil ; Zeng, Xi-Lei ; Atmar, Robert L. ; Estes, Mary K. ; Vinjé, Jan
• Description:
  Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
• Subjects:
  Alcohols Cell Line Chlorine Humans Jejunum Norovirus Research Virus Cultivation Virus Inactivation Virus Replication
• Source:
  Emerg Infect Dis. 24(8):1453-1464
• Pubmed ID:
  30014841
• Pubmed Central ID:
  PMC6056096
• Document Type:
  Journal Article
• Funding:
  P01 AI057788/AI/NIAID NIH HHS/United States ; P30 DK056338/DK/NIDDK NIH HHS/United States
• Volume:
  24
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:65a05769c8a838a2772072a10f70942cc5325f52afd14dc8a346827ffd6fe0b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/57967/cdc_57967_DS1.pdf
• File Type:
  [PDF - 3.14 MB ]