Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

Costantini, Veronica; Morantz, Esther K.; Browne, Hannah; Ettayebi, Khalil; Zeng, Xi-Lei; Atmar, Robert L.; Estes, Mary K.; Vinjé, Jan

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Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

Supporting Files

August 2018
By Costantini, Veronica ; Morantz, Esther K. ; Browne, Hannah
Series: Emerging Infectious Diseases

File Language:

English

Details

Journal Article:

Emerging Infectious Diseases
Personal Author:

Costantini, Veronica ; Morantz, Esther K. ; Browne, Hannah ; Ettayebi, Khalil ; Zeng, Xi-Lei ; Atmar, Robert L. ; Estes, Mary K. ; Vinjé, Jan
Description:

Human noroviruses are a leading cause of epidemic and endemic acute gastroenteritis worldwide and a leading cause of foodborne illness in the United States. Recently, human intestinal enteroids (HIEs) derived from human small intestinal tissue have been shown to support human norovirus replication. We implemented the HIE system in our laboratory and tested the effect of chlorine and alcohols on human norovirus infectivity. Successful replication was observed for 6 norovirus GII genotypes and was dependent on viral load and genotype of the inoculum. GII.4 viruses had higher replication levels than other genotypes. Regardless of concentration or exposure time, alcohols slightly reduced, but did not completely inactivate, human norovirus. In contrast, complete inactivation of the 3 GII.4 viruses occurred at concentrations as low as 50 ppm of chlorine. Taken together, our data confirm the successful replication of human noroviruses in HIEs and their utility as tools to study norovirus inactivation strategies.
Subjects:
Series:

Emerging Infectious Diseases
Pubmed ID:

30014841
Pubmed Central ID:

PMC6056096
Document Type:

Text ; Journal Article
Funding:

P01 AI057788/AI/NIAID NIH HHS/United States ; P30 DK056338/DK/NIDDK NIH HHS/United States
Genre:

Journal Article
Rights:

Public Domain
Volume:

24
Issue:

8
Citation:

Emerg Infect Dis. 24(8):1453-1464
Download URL:

https://stacks.cdc.gov/view/cdc/57967/cdc_57967_DS1.pdf
File Type:

[PDF - 3.14 MB]
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha-512:0c8a58c5238651e92340c71e90b95dff263c8c303bf2e09c4ff84089875004b98e0b680bb79d472ce93df3e207cfb6c365d46dc5106d1297460236682215ca0c

File Language:

English

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