Details:

Alternative Title:
J Clin Virol
Personal Author:
Chhabra, Preeti ; Gregoricus, Nicole ; Weinberg, Geoffrey A. ; Halasa, Natasha ; Chappell, James ; ... More +
Chhabra, Preeti ; Gregoricus, Nicole ; Weinberg, Geoffrey A. ; Halasa, Natasha ; Chappell, James ; Hassan, Ferdaus ; Selvarangan, Rangaraj ; Mijatovic-Rustempasic, Slavica ; Ward, M. Leanne ; Bowen, Michael ; Payne, Daniel C. ; Vinjé, Jan Less -
Description:
Background

Viruses are major etiological agents of childhood gastroenteritis. In recent years, several molecular platforms for the detection of viral enteric pathogens have become available.

Objective/study design

We evaluated the performance of three multiplex platforms including Biofire’s Gastrointestinal Panel (FilmArray), Luminex xTAG® Gastrointestinal Pathogen Panel (GPP), and the TaqMan Array Card (TAC) for the detection of five gastroenteritis viruses using a coded panel of 300 archived stool samples.

Results

The FilmArray detected a virus in 199 (96.1%) and the TAC in 172 (83.1%) of the 207 samples (187 samples positive for a single virus and 20 samples positive for more than one virus) whereas the GPP detected a virus in 100 (78.7%) of the 127 (97 positive for one virus and three positive for more than one virus) samples. Overall the clinical accuracy was highest for the FilmArray (98%) followed by TAC (97.2%) and GPP (96.9%). The sensitivity of the FilmArray, GPP and TAC platforms was highest for rotavirus (100%, 95.8%, and 89.6%, respectively) and lowest for adenovirus type 40/41 (97.4%, 57.9% and 68.4%). The specificity of the three platforms ranged from 95.6% (rotavirus) to 99.6% (norovirus/sapovirus) for the FilmArray, 99.6% (norovirus) to 100% (rotavirus/adenovirus) for GPP, and 98.9% (astrovirus) to 100% (rotavirus/sapovirus) for TAC.

Conclusion

The FilmArray demonstrated the best analytical performance followed by TAC. In recent years, the availability of multi-enteric molecular testing platforms has increased significantly and our data highlight the strengths and weaknesses of these platforms.


Subjects:
[+]
Adenovirus Type 40/41 Article Astrovirus Feces Gastroenteritis Gastroenteritis Viruses Humans Molecular Diagnostic Techniques Multiplex Polymerase Chain Reaction Norovirus Rotavirus Sapovirus Sensitivity And Specificity Virus Diseases
Source:
J Clin Virol. 95:66-71
Pubmed ID:
28889082
Pubmed Central ID:
PMC5940486
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U01 IP001049/IP/NCIRD CDC HHSUnited States/ ; U01 IP001063/IP/NCIRD CDC HHSUnited States/
CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U01 IP001049/IP/NCIRD CDC HHSUnited States/ ; U01 IP001063/IP/NCIRD CDC HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:83fa6734ea79ccaf8427a420575ab77fa98567909a7d6b1f85462acd579771af
Download URL:
https://stacks.cdc.gov/view/cdc/53832/cdc_53832_DS1.pdf
File Type:

Supporting Files

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