Phenotypic and Genotypic Characterization of Enterobacteriaceae Producing Oxacillinase-48–Like Carbapenemases, United States

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Lutgring, Joseph D. ; Zhu, Wenming ; de Man, Tom J.B. ; Avillan, Johannetsy J. ; Anderson, Karen F. ; Lonsway, David R. ; Rowe, Lori A. ; Batra, Dhwani ; Rasheed, J. Kamile ; Limbago, Brandi M.
• Description:
  Oxacillinase (OXA)-48-like carbapenemases remain relatively uncommon in the United States. We performed phenotypic and genotypic characterization of 30 Enterobacteriaceae producing OXA-48-like carbapenemases that were recovered from patients during 2010-2014. Isolates were collected from 12 states and not associated with outbreaks, although we could not exclude limited local transmission. The alleles β-lactamase OXA-181 (bla|) (43%), bla| (33%), and bla| (23%) were found. All isolates were resistant to ertapenem and showed positive results for the ertapenem and meropenem modified Hodge test and the modified carbapenem inactivation method; 73% showed a positive result for the Carba Nordmann-Poirel test. Whole-genome sequencing identified extended-spectrum β-lactamase genes in 93% of isolates. In all bla| isolates, the gene was on a ColKP3 plasmid. A total of 12 of 13 isolates harboring bla| contained the insertion sequence ΔISEcp1. In all isolates with bla|, the gene was located on a TN1999 transposon; these isolates also carried IncL/M plasmids.
• Subjects:
  Anti-Bacterial Agents Antimicrobial Resistance Bacteria Beta-Lactamases Enterobacteriaceae Genotypic Characterization Oxa-48 Oxacillinase-48–Like Carbapenemases Phenotypic And Genotypic Characterization Of Enterobacteriaceae Producing Oxacillinase-48–Like Carbapenemases, United States Phenotypic Characterization Research

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• Source:
  Emerg Infect Dis. 24(4):700-709.
• Pubmed ID:
  29553324
• Pubmed Central ID:
  PMC5875285
• Document Type:
  Journal Article
• Place as Subject:
  United States
• Location:
  North America
• Volume:
  24
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:dd33ece129a8f4900fdf537785b3521dd9de05862ee551b3a4aafb6ed77486c4
• Download URL:
  https://stacks.cdc.gov/view/cdc/53059/cdc_53059_DS1.pdf
• File Type:
  [PDF - 1.02 MB ]