Details:

Alternative Title:
Chem Biol Interact
Personal Author:
Marsillach, Judit ; Hsieh, Edward J. ; Richter, Rebecca J. ; MacCoss, Michael J. ; Furlong, Clement E.
Marsillach, Judit ; Hsieh, Edward J. ; Richter, Rebecca J. ; MacCoss, Michael J. ; Furlong, Clement E. Less -
Description:
Organophosphorus (OP) compounds include a broad group of toxic chemicals such as insecticides, chemical warfare agents and antiwear agents. The liver cytochromes P450 bioactivate many OPs to potent inhibitors of serine hydrolases. Cholinesterases were the first OP targets discovered and are the most studied. They are used to monitor human exposures to OP compounds. However, the assay that is currently used has limitations. The mechanism of action of OP compounds is the inhibition of serine hydrolases by covalently modifying their active-site serine. After structural rearrangement, the complex OP inhibitor-enzyme is irreversible and will remain in circulation until the modified enzyme is degraded. Mass spectrometry is a sensitive technology for analyzing protein modifications, such as OP-adducted enzymes. These analyses also provide some information about the nature of the OP adduct. Our aim is to develop high-throughput protocols for monitoring OP exposures using mass spectrometry.
Subject:
[+]
Agriculture
Article
Butyrylcholinesterase
Catalytic Domain
Cholinesterase Inhibitors
Environmental Exposure
Environmental Monitoring
High-Throughput Screening Assays
Humans
Mass Spectrometry
Occupational Exposure
Organophosphates
Organophosphorus Compounds
Protein Adducts
Proteomics
Serine
Tandem Mass Spectrometry

Source:
Chem Biol Interact. 2012; 203(1):85-90.
Pubmed ID:
23123252
Pubmed Central ID:
PMC4108156
Document Type:
Journal Article
Funding:
P41 GM103533/GM/NIGMS NIH HHS/United States ; R01 ES009883/ES/NIEHS NIH HHS/United States ; P42 ES004696/ES/NIEHS NIH HHS/United States ; 5P42ES04696/ES/NIEHS NIH HHS/United States ; R01 GM103551/GM/NIGMS NIH HHS/United States ; ... More +
P41 GM103533/GM/NIGMS NIH HHS/United States ; R01 ES009883/ES/NIEHS NIH HHS/United States ; P42 ES004696/ES/NIEHS NIH HHS/United States ; 5P42ES04696/ES/NIEHS NIH HHS/United States ; R01 GM103551/GM/NIGMS NIH HHS/United States ; NIEHS P30ES07033/ES/NIEHS NIH HHS/United States ; P30 ES007033/ES/NIEHS NIH HHS/United States ; ES09601/EPA-R826886/ES/NIEHS NIH HHS/United States ; P41GM103533/GM/NIGMS NIH HHS/United States ; P01 ES009601/ES/NIEHS NIH HHS/United States ; U54 OH007544/OH/NIOSH CDC HHS/United States ; R01ES09883/ES/NIEHS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:7bd58a362a6abff24c7e00a2082f2dc50a3d1e4cd2c33820181856849dc65faa
File Type:

Supporting Files

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