Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry1

Månsson, Viktor; Gilsdorf, Janet R.; Kahlmeter, Gunnar; Kilian, Mogens; Kroll, J. Simon; Riesbeck, Kristian; Resman, Fredrik

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Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry1

Supporting Files Public Domain

Mar 2018
By Månsson, Viktor ; Gilsdorf, Janet R. ; Kahlmeter, Gunnar ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Månsson, Viktor ; Gilsdorf, Janet R. ; Kahlmeter, Gunnar ; Kilian, Mogens ; Kroll, J. Simon ; Riesbeck, Kristian ; Resman, Fredrik
Description:

Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a-f and nontypeable; n = 258) and isogenic capsule transformants (types a-d) were investigated. Principal component and biomarker analyses of mass spectra showed clustering, and mass peaks correlated with capsule type-specific genetic lineages. We used 31 selected isolates to construct a capsule typing database. Validation with the remaining isolates (n = 227) showed 100% sensitivity and 92.2% specificity for encapsulated strains (a-f; n = 61). Blinded validation of a supplemented database (n = 50) using clinical isolates (n = 126) showed 100% sensitivity and 100% specificity for encapsulated strains (b, e, and f; n = 28). MALDI-TOF mass spectrometry is an accurate method for capsule typing of H. influenzae.
Subjects:

Bacterial Capsules/genetics Bacterial Typing Techniques Evolution, Molecular Genetic Linkage Haemophilus Infections/microbiology Haemophilus Influenzae/classification Haemophilus Influenzae/genetics Multilocus Sequence Typing Polymerase Chain Reaction Reproducibility Of Results Research Sensitivity And Specificity
Source:

Emerg Infect Dis. 24(3):443-452.
Pubmed ID:

29460728
Pubmed Central ID:

PMC5823330
Document Type:

Journal Article
Volume:

24
Issue:

3
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:770c30cd3e5ac0aa77bca0cc05aecd7664a1b179d14d4f618b3abdb3bd7da769
Download URL:

https://stacks.cdc.gov/view/cdc/52179/cdc_52179_DS1.pdf
File Type:

[PDF - 2.06 MB ]

File Language:

English

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