A high-throughput and rapid method for accurate identification of emerging multidrug-resistant Candida auris

Details

• Alternative Title:
  Mycoses
• Personal Author:
  Ahmad, Ausaf ; Spencer, Jonathan E. ; Lockhart, Shawn R. ; Singleton, Sabrina ; Petway, David J. ; Bagarozzi, Dennis A. ; Herzegh, Owen T.
• Description:
  Candida auris is an emerging multidrug-resistant yeast associated with invasive infection in healthcare settings. Recently, C auris cases in the United States have been detected in 11 states with the majority of cases in New York, New Jersey and Illinois. Rapid and accurate identification of C auris is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid DNA extraction method using the Roche MagNA Pure 96 instrument and a TaqMan real-time PCR assay for reliable, high-throughput identification of C auris. We evaluated 247 patient dermal swab samples previously analysed by culture/MALDI-TOF. The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 C auris CFU/10 μL. A receiver operating characteristic curve analysis of the real-time PCR data showed an area of 0.982 under the curve, with a C| cut-off value of ≤37.0. The turnaround time from DNA extraction to real-time PCR results was approximately 200 samples/day. In conclusion, we successfully validated a rapid and high-throughput method for accurate and reproducible identification of C auris with a significantly reduced turnaround time compared to culture/MALDI-TOF based methods.
• Keywords:
  Candida Candida Auris Candidiasis, Invasive Communicable Diseases, Emerging DNA, Fungal Drug Resistance, Multiple, Fungal High-throughput Rapid DNA Extraction Humans Molecular Diagnostic Techniques Real-Time Polymerase Chain Reaction Reproducibility Of Results ROC Curve Sensitivity And Specificity Specimen Handling TaqMan Real-time PCR Time Factors United States

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• Source:
  Mycoses. 62(6):513-518
• Pubmed ID:
  30801778
• Pubmed Central ID:
  PMC10888143
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  62
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5d62ec1a6bdeb3ed5b50c4cdd2e228b8a78e36db80c44fcc51172c53f692df00
• Download URL:
  https://stacks.cdc.gov/view/cdc/150332/cdc_150332_DS1.pdf
• File Type:
  [PDF - 881.26 KB ]