Simultaneous measurement of 3-chlorotyrosine and 3,5-dichlorotyrosine in whole blood, serum, and plasma by isotope dilution HPLC-MS/MS

Details

• Alternative Title:
  J Anal Toxicol
• Personal Author:
  Crow, Brian S. ; Quiñones-González, Jennifer ; Pantazides, Brooke G. ; Perez, Jonas W. ; Winkeljohn, W. Rucks ; Garton, Joshua W. ; Thomas, Jerry D. ; Blake, Thomas A. ; Johnson, Rudolph C.
• Description:
  Chlorine is a public health concern and potential threat due to its high reactivity, ease and scale of production, widespread industrial use, bulk transportation, massive stockpiles and history as a chemical weapon. This work describes a new, sensitive and rapid stable isotope dilution method for the retrospective detection and quantitation of two chlorine adducts. The biomarkers 3-chlorotyrosine (Cl-Tyr) and 3,5-dichlorotyrosine (Cl2-Tyr) were isolated from the pronase digest of chlorine exposed whole blood, serum or plasma by solid-phase extraction (SPE), separated by reversed-phase HPLC and detected by tandem mass spectrometry (MS-MS). The calibration range is 2.50-1,000 ng/mL (R2 ≥ 0.998) with a lowest reportable limit (LRL) of 2.50 ng/mL for both analytes, an accuracy of ≥93% and an LOD of 0.443 ng/mL for Cl-Tyr and 0.396 ng/mL for Cl2-Tyr. Inter- and intra-day precision of quality control samples had coefficients of variation of ≤10% and ≤7.0%, respectively. Blood and serum samples from 200 healthy individuals and 175 individuals with chronic inflammatory disease were analyzed using this method to assess background levels of chlorinated tyrosine adducts. Results from patients with no known inflammatory disease history (healthy) showed baseline levels of
• Subjects:
  Biomarkers Calibration Chromatography, High Pressure Liquid Humans Indicators And Reagents Inflammation Limit Of Detection Plasma Quality Control Radioisotope Dilution Technique Reproducibility Of Results Retrospective Studies Solid Phase Extraction Tandem Mass Spectrometry Tyrosine

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• Source:
  J Anal Toxicol. 40(4):264-271.
• Pubmed ID:
  26977104
• Pubmed Central ID:
  PMC5693347
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  40
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:24db15c77f33a886f42db888a5bd36a9a505df5425e9796fa762381f4ec1b2e1
• Download URL:
  https://stacks.cdc.gov/view/cdc/49923/cdc_49923_DS1.pdf
• File Type:
  [PDF - 593.56 KB ]