Details:

Alternative Title:
J Anal Toxicol
Personal Author:
Crow, Brian S.; Quiñones-González, Jennifer; Pantazides, Brooke G.; Perez, Jonas W.; Winkeljohn, W. Rucks; ... More +
Crow, Brian S.; Quiñones-González, Jennifer; Pantazides, Brooke G.; Perez, Jonas W.; Winkeljohn, W. Rucks; Garton, Joshua W.; Thomas, Jerry D.; Blake, Thomas A.; Johnson, Rudolph C.; Less -
Description:
Chlorine is a public health concern and potential threat due to its high reactivity, ease and scale of production, widespread industrial use, bulk transportation, massive stockpiles and history as a chemical weapon. This work describes a new, sensitive and rapid stable isotope dilution method for the retrospective detection and quantitation of two chlorine adducts. The biomarkers 3-chlorotyrosine (Cl-Tyr) and 3,5-dichlorotyrosine (Cl2-Tyr) were isolated from the pronase digest of chlorine exposed whole blood, serum or plasma by solid-phase extraction (SPE), separated by reversed-phase HPLC and detected by tandem mass spectrometry (MS-MS). The calibration range is 2.50-1,000 ng/mL (R2 ≥ 0.998) with a lowest reportable limit (LRL) of 2.50 ng/mL for both analytes, an accuracy of ≥93% and an LOD of 0.443 ng/mL for Cl-Tyr and 0.396 ng/mL for Cl2-Tyr. Inter- and intra-day precision of quality control samples had coefficients of variation of ≤10% and ≤7.0%, respectively. Blood and serum samples from 200 healthy individuals and 175 individuals with chronic inflammatory disease were analyzed using this method to assess background levels of chlorinated tyrosine adducts. Results from patients with no known inflammatory disease history (healthy) showed baseline levels of More ▼ -->
Subject:
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Article
Biomarkers
Calibration
Chlorinated Tyrosine
Chlorine Biomarkers
Chlorine Exposure
Chlorine Gas
Chromatography, High Pressure Liquid
Chronic Inflammation
Humans
Indicators And Reagents
Inflammation
Inflammatory Disease
LC-MS/MS
Limit Of Detection
Plasma
Quality Control
Radioisotope Dilution Technique
Reproducibility Of Results
Retrospective Studies
Solid Phase Extraction
Tandem Mass Spectrometry
Tyrosine

Source:
J Anal Toxicol. 40(4):264-271.;
Pubmed ID:
26977104
Pubmed Central ID:
PMC5693347
Document Type:
Journal Article;
Funding:
CC999999/Intramural CDC HHS/United States;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:24db15c77f33a886f42db888a5bd36a9a505df5425e9796fa762381f4ec1b2e1
File Type:

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